Why we recommend this TaqMan® Assay (Primer/Probe Set)

All TaqMan® Assays are designed using our industry-leading bioinformatics design process and meet all of our high quality control specifications. For the target you selected, we compared all assays for that target, and we recommend this assay for standard gene expression experiments because it detects the maximum number of transcripts for your gene of interest. We also evaluated the assays based on the following criteria, and the recommended assay best meets the design criteria below.

  1. Does not detect gene products with similar sequence (homologs).
  2. Is designed across an exon-exon junction.
  3. Is an inventoried product, so you get it faster.
  4. Has a short amplicon, giving you a more efficient PCR reaction.
  5. Does not detect off-target sequences, thus increasing the specificity of your reactions (off-target detection may happen when an assay hybridizes with a sequence that occurs more than once in the genome).
  6. Does not map to multiple genes, thus increasing the specificity of your experiment.
  7. Does not target the 5' untranslated region (UTR). The 5' UTR of transcripts can have variable sequence between transcripts.