Mm00658129_gH

• Gene Symbol: Pou5f1
• Gene Name: POU domain, class 5, transcription factor 1
• Gene Aliases: NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4
• Chromosome Location: Chr.17: 35506032 - 35510777 on Build GRCm38
• Species: Mouse
• Species Specific ID (Flybase ID): -

Assay Details

Gene Symbol: Pou5f1
Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	IMAGE Clone ID	Amplicon Length
RefSeq	NM_001252452.1	NP_001239381.1	2 - 3	208		148
	NM_013633.3	NP_038661.2	2 - 3	570		148
GenBank mRNA	AB221654.1	-	2 - 3	502		148
	AB375269.1	-	2 - 3	147		148
	AB375270.1	-	2 - 3	208		148
	AB375271.1	-	1 - 2	400		148
	AB375272.1	-	2 - 3	174		148
	AB375273.1	-	2 - 3	189		148
	AB375276.1	-	2 - 3	704		148
	AB375277.1	-	1 - 2	166		148
	AK145321.1	-	2 - 3	541		148
	BC068268.1	-	2 - 3	546	30019896	148
	M34381.1	-	2 - 3	551		148
	X52437.1	-	2 - 3	449		148

More Information

Additional Information:

Based on assay re-evaluation, this assay may detect genomic DNA. Please contact Tech Support if you need more information.

• Set Membership: Ambion siRNA available, Amplicon length greater than or equal to 101, Mammalian Gene Collection, Detect Genomic DNA, Mus musculus, Mouse Stem Cell Pluripotency, 1700, Inventoried, Mouse_Stem_Cell, Probe spans exons

Matching Ambion siRNAs:

Click here to find siRNAs for Gene Pou5f1

Gene Ontology Categories:

Process(es)

negative regulation of transcription from RNA polymerase II promoter
mesodermal cell fate commitment
endodermal cell fate commitment
ectodermal cell fate commitment
endodermal cell fate specification
trophectodermal cell differentiation
blastocyst growth
BMP signaling pathway involved in heart induction
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
response to organic substance
regulation of gene expression
stem cell population maintenance
germ-line stem cell population maintenance
response to retinoic acid
somatic stem cell population maintenance
positive regulation of catenin import into nucleus
mRNA transcription from RNA polymerase II promoter
cell fate commitment
negative regulation of cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of calcium ion-dependent exocytosis
stem cell differentiation
positive regulation of protein kinase B signaling
negative regulation of protein kinase B signaling
positive regulation of SMAD protein import into nucleus
cell fate commitment involved in formation of primary germ layer
cardiac cell fate determination
negative regulation of gene silencing by miRNA
regulation of heart induction by regulation of canonical Wnt signaling pathway
regulation of methylation-dependent chromatin silencing

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription coactivator activity
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
transcription factor binding
chromatin DNA binding
ubiquitin protein ligase binding
miRNA binding
sequence-specific DNA binding
transcription regulatory region DNA binding
poly(A) RNA binding
protein heterodimerization activity
POU domain binding
HMG box domain binding

MicroArray Probes:

Array Name	Probe IDs	Targets	Target Type
Affymetrix Mouse Clariom S Array	PSR1700007085.mm.1	NM_001252452.1.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007085.mm.1	NM_001252452.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007085.mm.1	NM_013633.3.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007085.mm.1	NM_013633.3	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007085.mm.1	TC1700000691.mm.1	MTA TC
Affymetrix Mouse Clariom S Array	PSR1700007090.mm.1	NM_001252452.1.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007090.mm.1	NM_001252452.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007090.mm.1	NM_013633.3.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007090.mm.1	NM_013633.3	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007090.mm.1	TC1700000691.mm.1	MTA TC
Affymetrix Mouse Clariom S Array	PSR1700007088.mm.1	NM_001252452.1.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007088.mm.1	NM_001252452.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007088.mm.1	NM_013633.3.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007088.mm.1	NM_013633.3	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007088.mm.1	TC1700000691.mm.1	MTA TC
Affymetrix Mouse Clariom S Array	PSR1700007075.mm.1	NM_013633.3.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007075.mm.1	NM_013633.3	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007075.mm.1	TC1700000691.mm.1	MTA TC
Affymetrix Mouse Clariom S Array	PSR1700007082.mm.1	NM_001252452.1.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007082.mm.1	NM_001252452.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007082.mm.1	TC1700000691.mm.1	MTA TC
Affymetrix Mouse Clariom S Array	PSR1700007087.mm.1	NM_001252452.1.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007087.mm.1	NM_001252452.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007087.mm.1	NM_013633.3.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007087.mm.1	NM_013633.3	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007087.mm.1	TC1700000691.mm.1	MTA TC
Affymetrix Mouse Clariom S Array	PSR1700007076.mm.1	NM_013633.3.1	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007076.mm.1	NM_013633.3	Refseq
Affymetrix Mouse Clariom S Array	PSR1700007076.mm.1	TC1700000691.mm.1	MTA TC

Array Name	Probe IDs	Targets	Target Type
ABI Mouse Genome Survey Microarray	615439	-	-