Rn00585470_s1 Best Coverage

• Gene Symbol: Twist1
• Gene Name: twist family bHLH transcription factor 1
• Gene Aliases: Twist
• Chromosome Location: Chr.6: 53401241 - 53403235 on Build Rnor_6.0
• Species: Rat
• Species Specific ID (Flybase ID): -

Assay Details

Gene Symbol: Twist1
Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	IMAGE Clone ID	Amplicon Length
RefSeq	NM_053530.2	NP_445982.1	1	531		71
GenBank mRNA	BC166412.1	-	1	661	9033082	71

More Information

Additional Information:

Based on assay re-evaluation, this assay may detect genomic DNA. Please contact Tech Support if you need more information.

• Set Membership: Ambion siRNA available, Amplicon length between 71 and 85, 3' Most, Mammalian Gene Collection, Detect Genomic DNA, Recommended Assay, Rattus norvegicus, Rat Tumor Metastasis Panel, 1700, Inventoried, Both primers and probe map within a single exon

Matching Ambion siRNAs:

Click here to find siRNAs for Gene Twist1

Panther Classification:

Molecular Function -

basic helix-loop-helix transcription factor

Gene Ontology Categories:

Process(es)

negative regulation of transcription from RNA polymerase II promoter
ossification
osteoblast differentiation
in utero embryonic development
neuron migration
neural tube closure
aortic valve morphogenesis
mitral valve morphogenesis
endocardial cushion morphogenesis
cardiac neural crest cell migration involved in outflow tract morphogenesis
transcription from RNA polymerase II promoter
positive regulation of gene expression
positive regulation of epithelial to mesenchymal transition
negative regulation of phosphatidylinositol 3-kinase signaling
cell differentiation
embryonic limb morphogenesis
regulation of bone mineralization
positive regulation of fatty acid beta-oxidation
negative regulation of tumor necrosis factor production
positive regulation of tumor necrosis factor production
negative regulation of histone phosphorylation
negative regulation of histone acetylation
embryonic forelimb morphogenesis
embryonic hindlimb morphogenesis
hindlimb morphogenesis
negative regulation of peroxisome proliferator activated receptor signaling pathway
odontogenesis
embryonic digit morphogenesis
negative regulation of apoptotic process
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of DNA damage response, signal transduction by p53 class mediator
negative regulation of molecular function
negative regulation of cell differentiation
negative regulation of osteoblast differentiation
negative regulation of striated muscle tissue development
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of skeletal muscle tissue development
embryonic cranial skeleton morphogenesis
embryonic skeletal system morphogenesis
positive regulation of epithelial cell proliferation
palate development
bone development
cranial suture morphogenesis
embryonic camera-type eye formation
eyelid development in camera-type eye
cardiac neural crest cell development involved in outflow tract morphogenesis
cellular response to growth factor stimulus
cellular response to hypoxia
positive regulation of monocyte chemotactic protein-1 production
positive regulation of cell motility
negative regulation of oxidative phosphorylation uncoupler activity
positive regulation of transcription regulatory region DNA binding
negative regulation of cellular senescence
positive regulation of interleukin-6 secretion
negative regulation of double-strand break repair
cell proliferation involved in heart valve development
positive regulation of endocardial cushion to mesenchymal transition involved in heart valve formation

Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding
molecular_function
transcription factor activity, sequence-specific DNA binding
transcription factor binding
protein domain specific binding
protein homodimerization activity
bHLH transcription factor binding
protein heterodimerization activity
E-box binding

MicroArray Probes:

Array Name	Probe IDs	Targets	Target Type
Agilent Whole Rat Genome Microarray 4x44K v2	A_44_P200042	-	-
Agilent SurePrint G3 Rat CGH Microarray 1x1M	688875	-	-
Agilent Whole Rat Genome Microarray 4x44K v3	A_44_P200042	-	-
Agilent Whole Rat Genome Microarray 4x44K v3	A_44_P200042	-	-
Agilent SurePrint G3 Rat GE 8x60K Microarray	A_44_P200042	-	-
SurePrint G3 Rat Gene Expression Microarray G4131A	A_44_P200042	-	-
SurePrint G3 Rat Gene Expression Microarray G4131A	A_44_P330417	-	-
Agilent Rat Genome CGH Microarray 105A (G4436A)	18515	-	-

Array Name	Probe IDs	Targets	Target Type
Affymetrix Rat Gene 1.0 ST Array	10884217	-	-
Affymetrix Rat Gene 1.0 ST Array	10884216	-	-
Affymetrix Rat Gene 1.1 ST Array	10884215	-	-
Rat Genome 230 2.0 Array	1387750_at	-	-
Rat Genome 230 2.0 Array	1378165_at	-	-
Rat Genome 230 2.0 Array	1378165_PM_at	-	-
Rat Genome 230 2.0 Array	1387750_PM_at	-	-
Affymetrix HT Rat Focus Array	1387750_at	-	-
Affymetrix HT Rat Focus Array	1378165_at	-	-
Affymetrix HT Rat Focus Array	1387750_PM_at	-	-
Affymetrix HT Rat Focus Array	1378165_PM_at	-	-
Affymetrix Rat Clariom S Assay	PSR0600004312.rn.1	TC0600000603.rn.1	RTA TC
Affymetrix Rat Clariom S Assay	PSR0600004311.rn.1	TC0600000603.rn.1	RTA TC
Affymetrix Rat Clariom S Assay	PSR0600004313.rn.1	TC0600000603.rn.1	RTA TC
Rat Expression Set 230	1387750_PM_at	-	-
Rat Expression Set 230	1387750_at	-	-
Rat Expression Set 230	1378165_PM_at	-	-
Rat Expression Set 230	1378165_at	-	-
Affymetrix Rat Gene 1.0 ST Array	10884215	-	-

Array Name	Probe IDs	Targets	Target Type
ABI Rat Genome Survey Microarray	21467836	-	-
ABI Rat Genome Survey Microarray	21511949	-	-

Array Name	Probe IDs	Targets	Target Type
Illumina ratRef-12 v1.0 expression beadchip	ILMN_1367694	-	-
RatRef-12 Expression BeadChip	ILMN_49649	-	-