Thermo Scientific™ 1-Step Human Coupled IVT Kit - DNA

Description

Rapidly express full-length, functional proteins from mRNA or plasmid templates with yields of up to 100μg/mL per reaction using these cell-free kits.

The Thermo Scientific™ 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100μg/mL per reaction.

The Human IVT Kits are unique HeLa cell lysate-based protein expression systems for in vitro translation or coupled transcription/translation reactions. Protein expression is performed in a single 90-minute reaction that can be extended for up to 6 hours with continued protein production up to 100μg/mL when combined with the optimized pT7CFE1 Expression Vector. The Human IVT Kits can express functional enzymes, phosphoproteins, glycoproteins and membrane proteins for immediate use in studying protein interactions, performing rapid mutational analysis and measuring activity.

Highlights:

Functional – uses the human translational machinery to express active proteins

• Convenient – perform transcription and translation in a single step
• High performance – greater yields compared to rabbit reticulocyte in vitro translation
• Reliable – express proteins that fail in rabbit reticulocyte systems

Better Than Traditional Methods:

• HeLa cell-free extracts are capable of expressing proteins with post-translational modifications
• Accurate translation delivers full-length protein compatible with downstream applications
• Protein translation is optimized with EMCV IRES element and other mRNA stabilizing elements

Includes:

Complete kits contain HeLa cell lysate, accessory proteins, reaction mix, nuclease-free water, expression vector and a GFP-positive control vector

Requires:

30°C incubator or water bath

Recommended for:

Express proteins to measure enzyme activity; Perform mutational analysis; Express protein for use in gel mobility shift assays; Perform co-immunoprecipitation; Express cytotoxic proteins; Perform unnatural amino acid labeling

Specifications

• Description: Pierce 1-Step Human Coupled IVT Kit - DNA
• Type: 1-step Human Coupled IVT Kit
• Sufficient For: 8 Reactions of 25 μL Each
• Quantity: 8 Reaction Kit
• Content And Storage: Sufficient For: 8 reactions of 25 μL each
  • HeLa Lysate, 110 μL
  • Accessory Proteins, 25 μL
  • Reaction Mix, 40 μL
  • Positive Control DNA: pCFE-GFP, 10 μg
  • pT7CFE1-CHis, 10 μg
  • Nuclease-Free Water, 1.5 mL
  
  Upon receipt store kit at -80°C. Product is shipped on dry ice
• Expression System: Cell-Free
• Expressed Protein Type: Membrane Protein

For Research Use Only. Not for use in diagnostic procedures.