Thermo Scientific™ 1-Step Human High-Yield IVT Kit

Express functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems with Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits.

The Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits with continuous-feed device enables the expression of functional proteins with 10 to 100 times greater yield per mL than other eukaryotic IVT translation systems.

The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human transcription and translation machinery and generate functional full-length proteins. In this system, protein expression is performed in a proprietary mini dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression for up to 24 hours and produces protein yields ranging from 250 to 750μg/mL. The complete kits come with all components required for proper transcription and translation of recombinant protein, including an optimized expression vector.

Highlights:

• High expression – up to 750μg/mL of expressed protein

• Reproducibility – low variability between experiments

• Fast – express high levels of protein with an overnight incubation

• Functional – obtain more active protein than with bacterial protein expression systems

1-Step Protein Expression:

• Streamlined reaction – requires only five components

• Easy-to-use – transcription and translation is performed in one reaction step

• Adapt for labeling – amenable to incorporation of heavy or unnatural amino acids

Includes:

Complete kits contain cell lysate, reaction mix, accessory proteins, 5X dialysis buffer, control DNA, cloning vector, mini dialysis devices and centrifuge tubes

Requires:

30°C incubator or water bath

Recommended for:

Production of recombinant proteins for structural analysis; Generate active proteins for protein interaction studies; Perform rapid expression and characterization of mutant proteins; Small-scale production of virual particles; Incorporation of unnatural amino acids or toxic protein production

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Type

1-step Human High-yield Mini IVT Kit

Sufficient For

10 Reactions of 100 μL Each

Quantity

10 x 100 μL

Content And Storage

Sufficient For: 10 reactions of 100 μL each
• HeLa Lysate, 500 μL
• Accessory Proteins, 100 μL
• Reaction Mix, 200 μL
• 5X Dialysis Buffer, 3 mL
• Positive Control DNA (pCFE-GFP), 10 μg
• pT7CFE1-NHis-GST-CHA, 10 μg
• Microdialysis Device, 10 each
• Nuclease-free Water, 3 x 5 mL

Store micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C.

Expression System

Cell-Free

Expressed Protein Type

Insoluble Protein

For Research Use Only. Not for use in diagnostic procedures.