Thermo Scientific™ 1-Step Human High-Yield IVT Kit

Description

Express functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems with Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits.

The Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits with continuous-feed device enables the expression of functional proteins with 10 to 100 times greater yield per mL than other eukaryotic IVT translation systems.

The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human transcription and translation machinery and generate functional full-length proteins. In this system, protein expression is performed in a proprietary mini dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression for up to 24 hours and produces protein yields ranging from 250 to 750μg/mL. The complete kits come with all components required for proper transcription and translation of recombinant protein, including an optimized expression vector.

Highlights:

• High expression – up to 750μg/mL of expressed protein

• Reproducibility – low variability between experiments

• Fast – express high levels of protein with an overnight incubation

• Functional – obtain more active protein than with bacterial protein expression systems

1-Step Protein Expression:

• Streamlined reaction – requires only five components

• Easy-to-use – transcription and translation is performed in one reaction step

• Adapt for labeling – amenable to incorporation of heavy or unnatural amino acids

Includes:

Complete kits contain cell lysate, reaction mix, accessory proteins, 5X dialysis buffer, control DNA, cloning vector, mini dialysis devices and centrifuge tubes

Requires:

30°C incubator or water bath

Recommended for:

Production of recombinant proteins for structural analysis; Generate active proteins for protein interaction studies; Perform rapid expression and characterization of mutant proteins; Small-scale production of virual particles; Incorporation of unnatural amino acids or toxic protein production

Specifications

• Type: 1-step Human High-yield Mini IVT Kit
• Sufficient For: 2 Reactions of 100 μL Each
• Quantity: 2 x 100 μL
• Content And Storage: Sufficient For: 2 reactions of 100 μL each
  • HeLa Lysate, 110 μL
  • Accessory Proteins, 25 μL
  • Reaction Mix, 40 μL
  • 5X Dialysis Buffer, 600 μL
  • Positive Control DNA (pCFE-GFP), 10 μg
  • pT7CFE1-NHis-GST-CHA, 10 μg
  • Microdialysis Device, 2 each
  • Nuclease-free Water, 5 mL
  
  Store micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C.
• Expression System: Cell-Free
• Expressed Protein Type: Insoluble Protein

For Research Use Only. Not for use in diagnostic procedures.