Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

3,3',5,5'-Tetramethylbenzidine, 98%, Thermo Scientific Chemicals

Catalog No. AAA1386806
Click to view available options
Quantity:

3,3',5,5'-Tetramethylbenzidine, 98%, or TMB, CAS # 54827-17-7, is a colorimetric reagent used in laboratory-based substrate detection.

Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes; particularly useful in ELISA techniques, blue result can be read spectrophotometrically at 370 or 650 nm3,3',5,5'-Tetramethylbenzidine is used as a clinical reagent for testing blood. It is also a chromogenic substrate which is used in staining procedures in immunohistochemistry. Further, it is used as a visualizing reagent in enzyme-linked immunosorbent assays. In addition to this, it is used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description
• 3,3',5,5'-Tetramethylbenzidine, also known as 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl or TMB, is a colorimetric substrate for detection of horseradish peroxidase-labeled probes
• Oxidation of 3,3',5,5'-Tetramethylbenzidine results in a pale blue end product that can be detected at 370 or 655 nm. Sulfuric acid (H2SO4) is used to stop the oxidation reaction and stabilize the reading, resulting in a yellow color that can be read at 450 nm
Application
• 3,3',5,5'-Tetramethylbenzidine is used as a chromogenic visualization agent in enzyme-linked immunosorbent assays (ELISA) or immunohistochemistry (IHC) techniques
• 3,3',5,5'-Tetramethylbenzidine has been used to detect molecules in aqueous samples such as copper ion and hypochlorite

Chemical Identifiers

CAS

54827-17-7

Molecular Formula

C16H20N2

Molecular Weight (g/mol)

240.35

MDL Number

MFCD00007748

InChI Key

UAIUNKRWKOVEES-UHFFFAOYSA-N

Synonym

3,3',5,5'-tetramethylbenzidine, 3,3',5,5'-tetramethyl-1,1'-biphenyl-4,4'-diamine, 3,5,3',5'-tetramethylbenzidine, tmb, 3,3',5,5'-tetramethyl benzidine, tmb substrate, bm blue, ccris 4727, tmb plus, tmb, insoluble

PubChem CID

41206

IUPAC Name

4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline

SMILES

CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C

Specifications

Melting Point

168°C to 171°C

Quantity

5 g

Beilstein

2808541

Sensitivity

Light and moisture sensitive

Solubility Information

Soluble in ethyl acetate,ethanol,acetone,chloroform,toluene and dimethyl sulfoxide. Insoluble in water.

Formula Weight

240.35

Percent Purity

98%

Chemical Name or Material

3,3',5,5'-Tetramethylbenzidine

Hazard Category

H315-H319-H332-H335-H341

Hazard Statement

GHS H Statement
H302-H315-H319-H335
Harmful if swallowed.
Causes skin irritation.
Causes serious eye irritation.
May cause respiratory irritation.

Precautionary Statement

P201-P202-P261-P264-P264b-P270-P271-P280-P301+P312-P302+P352-P304+P340-P305+P351+P338-P308+P313-P312-P330-P332+P313-P362-P501c

EINECSNumber

259-364-6

RTECSNumber

DV2300000

TSCA

Yes

Recommended Storage

Ambient temperatures; Store under Nitrogen

RUO – Research Use Only

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.