3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide, MP Biomedicals™

Description

Used for measurement of cell division and proliferation. 3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide, MP Biomedicals (MTT) is a histochemical/cytochemical reagent. It is converted to water-insoluble MTT-formazan of a dark blue color by mi

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. It has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.

• MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells
• The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570nm
• MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems

Specifications

Chemical Identifiers

• CAS: 298-93-1
• Molecular Formula: C18H16BrN5S
• Molecular Weight (g/mol): 414.33
• MDL Number: MFCD00011964,MFCD00066662
• InChI Key: AZKSAVLVSZKNRD-UHFFFAOYSA-M
• Synonym: thiazolyl blue, thiazolyl blue tetrazolium bromide, mmt tetrazolium, methylthiazoletetrazolium, mtt, thiazolyl blue monotetrazolium, thiazole blue, 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide, mtt van, unii-euy85h477i
• PubChem CID: 64965
• ChEBI: CHEBI:53233
• IUPAC Name: 2-(4,5-dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-3H-1,2λ⁵,3,4-tetrazol-2-ylium bromide
• SMILES: [Br-].CC1=C(C)N=C(S1)[N+]1=NC(=NN1C1=CC=CC=C1)C1=CC=CC=C1

• Absorbance: 376 ± 2nm; 242 ± 2nm
• Melting Point: 189°C to 199°C
• Quantity: 250mg
• Solubility Information: Soluble in methyl alcohol (heat), methanol (10mg/mL with heat, clear, yellow solution) and water (5mg/mL, clear to very slightly hazy, yellow to bright yellow solution).
• Formula Weight: 414.3
• Physical Form: Yellow Powder
• Percent Purity: ∼98%

Safety and Handling

Recommended Storage

+4°C, protect from light