3,5-Dinitrosalicylic acid, 98%, Thermo Scientific Chemicals

3,5-dinitrosalicylic acid, CAS # 609-99-4, also known as DNS or DNSA, is used for quantifying carbohydrate levels in blood by calorimetry.

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

3,5-Dinitrosalicylic acid also known as DNA or DNSA reacts with reducing sugars forming 3-amino-5-nitrosalicylic acid, which is an aromatic compound with maximum absorption at 540 nm allowing spectrophotometer measurement of the amount of reducing molecules

Applications

• It is used in the quantification of reducing sugars by colorimetry in the blood. It is also used to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar
• 3,5-Dinitrosalicylic acid can detect reducing substances in the urine
• This compound is used to detect the presence of free carbonyl groups (C=O)
• 3,5-Dinitrosalicylic acid can be used to determine the effects of silver nanoparticles (AgNPs) on membrane leakage

Chemical Identifiers

CAS

609-99-4

Molecular Formula

C₇H₄N₂O₇

Molecular Weight (g/mol)

228.12

MDL Number

MFCD00007104

InChI Key

LWFUFLREGJMOIZ-UHFFFAOYSA-N

Synonym

3,5-dinitrosalicylic acid, 2-hydroxy-3,5-dinitrobenzoic acid, 3,5-dinitro-2-hydroxybenzoic acid, 3,5-dinitrosalicylate, benzoic acid, 2-hydroxy-3,5-dinitro, salicylic acid, 3,5-dinitro, dnsa, o-dncp, o-dinitrocarboxylphenol, 3,5-dinitro-2-salicylic acid

PubChem CID

11873

ChEBI

CHEBI:53648

SMILES

C1=C(C=C(C(=C1[N+](=O)[O-])O)C(=O)O)[N+](=O)[O-]

CAS Min %

97.5

CAS Max %

100.0

Melting Point

170.0°C to 174.0°C

Color

Yellow

Infrared Spectrum

Authentic

Assay Percent Range

98%

Packaging

Glass bottle

Linear Formula

(O₂N)₂C₆H₂(OH)CO₂H

Quantity

1 kg

Beilstein

10,122

Solubility Information

Solubility in water: soluble. Other solubilities: soluble in alcohol and benzene

Formula Weight

228.12

Percent Purity

98%

Physical Form

Powder

Chemical Name or Material

3,5-Dinitrosalicylic acid, 98%

Hazard Category

GHS Signal Word: Warning

Hazard Statement

GHS H Statement
Harmful if swallowed.
Causes skin irritation.
Causes serious eye irritation.
May cause respiratory irritation.

Precautionary Statement

GHS P Statement
Wear protective gloves/protective clothing/eye protection/face protection.
IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing.
IF SWALLOWED: rinse mouth.
Do NOT induce vomiting.

EINECSNumber

210-204-3

RTECSNumber

VO2965000

TSCA

TSCA

SDS

Hazard Category

GHS Signal Word: Warning

Hazard Statement

GHS H Statement
Harmful if swallowed.
Causes skin irritation.
Causes serious eye irritation.
May cause respiratory irritation.

Precautionary Statement

GHS P Statement
Wear protective gloves/protective clothing/eye protection/face protection.
IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing.
IF SWALLOWED: rinse mouth.
Do NOT induce vomiting.

EINECSNumber

210-204-3

RTECSNumber

VO2965000

TSCA

TSCA

SDS

RUO – Research Use Only