Applied Biosystems™ 7500 Fast Real-Time PCR System, tower, QST

The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility.

Supplier:  Applied Biosystems™ 4365463

Catalog No. 43-654-63

Description

It is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

NEW - 7500 Software v2.0:

Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.0 upgrade. The 7500 Software v2.0 incorporates your favorite StepOne Software features, such as multiple plate setup wizards, calculators for standard curve dilution and master mix recipes, QCX3 flags, data filters, and email notification when a run is finished. The 7500 Software v2.0 also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates.

 

The NEW Gene Expression Study package accommodates large studies better than any other instrument software package:

• Import an unlimited number of Comparative CT (relative quantitation) files to one study
• Group samples and view data both by technical replicate group and biological replicate group
• Use any gene(s) as an endogenous control, including averaging multiple controls together
• Enter known efficiency values to be factored into the RQ results

 

21 CFR Part 11 Module available:

The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.

• Individual user log-ins can be added for up to four user groups, each group with designated permission settings
• User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
• Audit trails can be enabled or disabled depending on your traceability needs
• A selection of e-signatures is available to ease e-signatures into your workflow

 

Supports Many Applications:

Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls. To facilitate many of these applications, we provide preformulated, ready-to-use, quality-tested, TaqMan™ assays for use with the 7500 system. Now you can reduce your assay optimization efforts.

 

Upgrade to High-Speed Thermal Cycling:

An optional upgrade to a the 7500 Fast System is available. This 7500 Fast System uses our master mix formulations and enables you to shorten your real-time PCR runs to as little as 30 minutes.

 

For Research Use Only. Not for use in diagnostics procedures.

Specifications

• Calibrated Dye: SYBR™ Green I, ROX™, NED™, TAMRA™, JOE™, Texas Red™, Cy5™, VIC™, FAM™, Cy3™
• Includes: Tower
• Dynamic Range: 9 logs of linear dynamic range
• Format: 96-well plate
• High-throughput Compatibility: High-throughput Compatible, Multiplexing
• Optics: Tungsten-halogen lamp, five-emission filters and CCD camera, Five-excitation filters
• Precision: Can distinguish between 5000 and 10000 template copies with 99.7% confidence
• Sample Ramp Rate: Fast mode: ± 3.5°C/sec., Standard mode: ± 1.6°C/sec.
• Software Compatibility: Primer Express Software, System Software
• Temperature Range (Metric): 4° to 100°C
• Thermal Cycling System: Peltier-Based System
• Dimensions: 34 cm (W) x 49 cm (H) x 45 cm (D) (13.39 x 19.29 x 17.72 in.)
• Voltage: 120 V

• Data Acquisition: Collects data for all 5 filters for all wells regardless of plate setup
• Reaction Speed: Fast, Standard
• External Computer: Minitower
• For Use With (Equipment): 7500 Fast System
• Max. Power: 950 W
• Passive Reference Dye: ROX (Separate Tube), ROX (Pre-mixed)
• Reaction Volume Range: 10-30 µL
• Sensitivity: Down to 1 copy of RNase P gene from human genomic DNA
• Temperature Accuracy: Within 0.5°C (35° to 95°C) of setpoint/display temp (3 min. after start)
• Temperature Uniformity: Within 1.0°C (30 seconds after clock start)
• Throughput: Manual loading of four-five 96-well plates per 8-hr day (real-time throughput)
• Weight (English): 75 lb.