Gibco™ AAV-MAX Helper-Free AAV Production System Kit

The Gibco AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production.

Supplier:  Gibco™ A51217

Catalog No. A51217

Description

The Gibco AAV-MAX Helper-Free AAV Production System is based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium. Optimal viral titers are mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel production enhancer. The components are designed to work synergistically, resulting in maximal titers and eliminating the need to optimize reagents and protocols.

Features of the AAV-MAX Helper-Free AAV Production System include:

• Components work synergistically to produce high AAV titers greater than 5 x 10¹⁰ vg/mL (unpurified)
• Suspension-based system has scalable protocols from shake flask to bioreactor scale
• Simplified workflow—streamlined protocol using helper virus-free triple transfection
• Clonal, 293F-derived, high-producer cell line
• Robust transfection protocols are adaptable to large-scale transfection

The components included in the AAV-MAX System Kit are:

• 1 vial of Viral Production Cells 2.0
• 1 L of Viral Production Medium
• 1 AAV-MAX Transfection Kit for 1 L of production
• 100 mL Viral-Plex Complexation Buffer
• 100 mL AAV-MAX Lysis Buffer

All components are also available for purchase separately.

Specifications

• Content And Storage: • 1 vial Viral Production Cells 2.0
  • 1 L Viral Production Medium
  • 1 AAV-MAX Transfection Kit for 1 L production
  • 100 mL Viral-Plex Complexation Buffer
  • 100 mL AAV-MAX Lysis Buffer
  • 100 mL GlutaMAX Supplement
  
  Storage conditions vary
  
  
• Cell Type: Kidney (Embryonic)
• Product Type: AAV Production System
• For Use With (Application): AAV Production
• Quantity: For 1 L of culture

• Cell Line: Viral Production Cells 2.0
• Culture Type: Suspension Cell Culture
• Sterility: Sterile
• Product Line: AAV-MAX

Safety and Handling

ShelfLife : 12 months from date of manufacture

For Research Use Only. Not for use in diagnostic procedures.