Gibco™ AAV-MAX Helper-Free AAV Production System Kit

The Gibco AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production.

The Gibco AAV-MAX Helper-Free AAV Production System is based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium. Optimal viral titers are mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel production enhancer. The components are designed to work synergistically, resulting in maximal titers and eliminating the need to optimize reagents and protocols.

Features of the AAV-MAX Helper-Free AAV Production System include:

• Components work synergistically to produce high AAV titers greater than 5 x 10¹⁰ vg/mL (unpurified)
• Suspension-based system has scalable protocols from shake flask to bioreactor scale
• Simplified workflow—streamlined protocol using helper virus-free triple transfection
• Clonal, 293F-derived, high-producer cell line
• Robust transfection protocols are adaptable to large-scale transfection

The components included in the AAV-MAX System Kit are:

• 1 vial of Viral Production Cells 2.0
• 1 L of Viral Production Medium
• 1 AAV-MAX Transfection Kit for 1 L of production
• 100 mL Viral-Plex Complexation Buffer
• 100 mL AAV-MAX Lysis Buffer

All components are also available for purchase separately.

Content And Storage

• 1 vial Viral Production Cells 2.0
• 1 L Viral Production Medium
• 1 AAV-MAX Transfection Kit for 1 L production
• 100 mL Viral-Plex Complexation Buffer
• 100 mL AAV-MAX Lysis Buffer
• 100 mL GlutaMAX Supplement

Storage conditions vary

Cell Line

Viral Production Cells 2.0

Cell Type

Kidney (Embryonic)

Culture Type

Suspension Cell Culture

Product Type

AAV Production System

Sterility

Sterile

For Use With (Application)

AAV Production

Product Line

AAV-MAX

Quantity

For 1 L of culture

For Research Use Only. Not for use in diagnostic procedures.