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- An essential component of the AAV-MAX Helper-Free AAV Production System
- It is formulated for the transient transfection of Viral Production Cells 2.0
- The kit includes a cationic lipid-based transfection reagent, as well as a transfection booster and production enhancer
- These aspects are designed to work together to produce adeno-associated virus (AAV) particles from Viral Production Cells 2.0 when cultured in Viral Production Medium
Features of the AAV-MAX Transfection Kit include:
- Components work synergistically within the AAV-MAX system to produce high AAV titers greater than 5 x 1010 vg/mL (unpurified)
- AAV-MAX transfection reagent - cationic lipid-based transfection reagent for helper virus-free triple transfection of high-density cultures
- AAV-MAX booster - chemically defined component that works with transfection reagent to achieve high transfection efficiency
- AAV-MAX enhancer - chemically defined component that enhances the production of AAV particles
- Robust transfection protocols are adaptable to large scale transfection
Specifications
Cell Line
HEK293
Content And Storage
• 6 mL AAV-MAX Trasfection Reagent
• 3 mL AAV-MAX Transfection Booster
• 10 mL AAV-MAX Enhancer
Reagents should be stored at 2–8°C. Do not freeze.
Culture Type
Suspension Cell Culture
Description
Serum-free, animal origin-free, chemically defined
Cell Type
Established Cell Lines
Format
Bottle
Sample Type
Plasmid DNA
Transfection Technique
Lipid-based Transfection
Expression System
Mammalian
For Use With (Equipment)
AAV-MAX System
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