Abnova™ SREBF2 Transcription Factor Assay Kit

SREBF2 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.

• A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA)
• A specific double stranded DNA (dsDNA) sequence containing the SREBP response element is immobilized onto the wells of a 96-well plate
• SREBP contained in a nuclear extract, binds specifically to the SREBP response element
• SREBP is detected by addition of a specific primary antibody directed against SREBP
• A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm
• This product is for laboratory research use only: not for administration to humans
• This kit will perform as specified if stored as directed and used before the expiration date indicated on the outside of the box

Description

SREBF2 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.

Immunoassay Kit Format

ELISA (Standard)

Product Type

SREBF2 Transcription Factor Assay Kit

Sufficient For

15 Reactions

For Use With (Application)

Functional Study

Gene ID (Entrez)

6721

Gene Symbol

SREBF2

Kit Contents

Transcription factor antibody binding buffer (10X), Transcription factor binding assay buffer (4X), Transcription factor reagent A, Transcription factor SREBP-2 positive control, Transcription factor SREBP-2 primary antibody, Wash buffer concentrate

Quantity

1 Kit

Storage Requirements

Store the kit at -20°C temporarily. For best results, store the components as described in the protocol.