Abnova™ SREBF2 Transcription Factor Assay Kit

Description

SREBF2 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.

• A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA)
• A specific double stranded DNA (dsDNA) sequence containing the SREBP response element is immobilized onto the wells of a 96-well plate
• SREBP contained in a nuclear extract, binds specifically to the SREBP response element
• SREBP is detected by addition of a specific primary antibody directed against SREBP
• A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm
• This product is for laboratory research use only: not for administration to humans
• This kit will perform as specified if stored as directed and used before the expiration date indicated on the outside of the box

Specifications

• Description: SREBF2 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
• Immunoassay Kit Format: ELISA (Standard)
• Product Type: SREBF2 Transcription Factor Assay Kit
• Sufficient For: 15 Reactions
• For Use With (Application): Functional Study
• Gene ID (Entrez): 6721
• Gene Symbol: SREBF2
• Kit Contents: Transcription factor antibody binding buffer (10X), Transcription factor binding assay buffer (4X), Transcription factor reagent A, Transcription factor SREBP-2 positive control, Transcription factor SREBP-2 primary antibody, Wash buffer concentrate
• Quantity: 1 Kit
• Storage Requirements: Store the kit at -20°C temporarily. For best results, store the components as described in the protocol.