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Thermo Scientific™ Acclaim™ Mixed Mode HILIC-1 HPLC Columns

Catalog No. p-4523509 Shop All Thermo Scientific Products
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Length (Metric):
10 mm
50 mm
150 mm
250 mm
Diameter (Metric):
2.1 mm
3 mm
4.6 mm
Particle Size:
3 μm
5 μm

Get all the advantages of reversed-phase and hydrophilic interaction liquid chromatography (HILIC) in one column with the Thermo Scientific™ Acclaim™ Mixed-Mode HILIC-1 column.

Get all the advantages of reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) in one column with the Thermo Scientific™ Acclaim™ Mixed-Mode HILIC-1 column. The high-efficiency, silica-based, mixed-mode stationary phase consists of a hydrophobic alkyl chain with a diol group at the terminus. The hydrophobic moiety supplies reversed-phase retention and the terminal diol group facilitates hydrophilic interactions. This unique combination provides adjustable selectivity with unsurpassed resolution and peak symmetry for a variety of both polar and nonpolar molecules.

Separates Both Polar and Nonpolar Molecules with Selectivity Complementary to RP Columns
  • Operates in both RP and HILIC modes
  • Retains highly polar molecules
  • Covers broader application range than conventional diol-based columns, including nonionic ethoxylated surfactants, drug metabolites, lipids, and polyethylene glycols (PEGs)
  • Stable between pH 2.5 and 7.5
  • Available in 3 and 5μm particle sizes with an average pore diameter of 120Å

Specifications

Column Format Analytical Column
Diameter (Metric) 4.6 mm
Length (Metric) 150 mm
Max. Pressure 5800 psi (400 bar)
Particle Size 5 μm
pH 2.5 to 7.5
Pore Size 120 Å
Surface Area 300 m²/g
Temperature 50°C
USP Type N/A
Stationary Phase Hydrophobic Alkyl Chain with a Diol Group
Product Line Acclaim
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Mixed Mode
Endcapped Proprietary
Phase Reversed Phase and HILIC
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