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Vector Laboratories Wheat Germ Agglutinin (WGA) Agarose Bound 10 ml

Wheat germ agglutinin (WGA) is a group of closely related isolectins. WGA can bind to oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues. This lectin can be used for the purification of insulin receptors and for neuronal tracing. Agarose Bound WGA is prepared using affinity-purified lectins and heat stable, cross-linked 4% agarose beads. The attachment of the lectins through a hydrophilic spacer arm to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins. The recommended inhibiting/eluting sugar is chitin hydrolysate or 500 mM N-acetylglucosamine. Salt and/or acid elution may also be required.
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