Edvotek™ AIDS Kit I: Simulation of HIV-1 Detection

Includes

Complete instructions, background information, and study questions; biologicals and reagents; three microtiter plates; 50 transfer pipettes; 60 microtest tubes with attached caps; twenty-five 1mL pipettes; two 50mL plastic tubes.

Requires

Requirements:

• Distilled or deionized water
• Beakers z 37°C incubation oven
• Automatic micro- pipettes and tips (optional)

In this experiment, students will perform an ELISA test by coating microtiter plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

A model for HIV-1 detection without the use of blood

Simulates the screening for antibodies against human immunodeficiency virus (HIV-1). Enzyme-linked immunoabsorbent assay (ELISA) is currently used for initial clinical screening of the blood supply for HIV-1. Students add simulated HIV antigen to wells in the microtiter plate and follow it with washing and introduction of simulated antibodies of HIV-1 positive or negative samples. A second antibody is added which binds to the first antibody of HIV-1 positive samples and results in a color change.

Note: some kit contents may need to be redeemed by mail.

Order Info

Kit ships with plasticware and instructions on how to obtain remaining materials directly from the supplier, Edvotek.

Product Type

AIDS Kit I

For Use With (Application)

Detects hiv infection indirectly using an elisa test against hiv antibodies in the blood

Includes

Instructions, simulated hiv antigens, serum samples, antibodies, buffers, microtiter plates, assorted pipets and microtest tubes

Time Range

60 min.

Content And Storage

Refrigerated

Class Size

10 Lab Groups