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Molecular Probes™ Alexa Fluor™ 594 Microscale Protein Labeling Kit
SDP

Catalog No. A30008
Encompass
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1 kit

Alexa Fluor™ 594 Microscale Protein Labeling Kit

Microscale Protein Labeling Kits provide a convenient means for attaching a fluorescent label to a small amount of antibody or protein (20–100µg). The kits are available in four Alexa Fluor™ colors (or biotin) and supply everything needed for three labeling and separation reactions.

Important Features of Microscale Protein Labeling Kits:
  • Labeled proteins typically ready to use typically in 2 hours (∼30 minutes hands-on time)
  • Optimized for 20–100µg of protein with molecular weights between 12 and 150 kDa
  • Purified using convenient spin filters with yields between 60 and 90%
  • Stabilizing proteins must be removed from the sample before labeling

    Stable Reaction Chemistry and Superior Alexa Fluor™ Dyes
    In the Microscale Protein Labeling Kits, the reactive dye contains a succinimidyl (NHS) ester moiety that reacts with primary amines of proteins to form stable dye-protein conjugates. Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. And generally when using Alexa Fluor™ dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor™ Dyes Spanning the Visible and Infrared Spectrum—Section 1.3.

    Learn More About Protein and Antibody Labeling
    We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See Antibody Labeling from A to Z or use our Labeling Chemistry Selection Tool for other choices. To learn more about our various kits read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in the Molecular Probes™ Handbook.

    We’ll Make a Custom Antibody Conjugate for You
    If you can’t find what you’re looking for in our stocked list, we’ll prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

    For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
  • Specifications

    Product Type Labeling Kit
    Quantity 1 kit
    Content And Storage Store in refrigerator 2°C to 8°C and protect from light.
    Detection Method Fluorescence
    Excitation/Emission 590/617 nm
    Labeling Method Conjugation-based
    Labeling Scale 20–100 μg
    Shipping Condition Room Temperature
    Product Line Alexa Fluor™
    Reactive Moiety Succinimidyl (NHS) Ester
    Label or Dye Alexa Fluor 594
    Color Red
    Labeling Target Proteins
    Label Type Alexa Fluor
    Show More Show Less

    For Research Use Only. Not for use in diagnostic procedures.

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