Molecular Probes™ Alexa Fluor™ 594 Microscale Protein Labeling Kit

Alexa Fluor™ 594 Microscale Protein Labeling Kit

Microscale Protein Labeling Kits provide a convenient means for attaching a fluorescent label to a small amount of antibody or protein (20–100µg). The kits are available in four Alexa Fluor™ colors (or biotin) and supply everything needed for three labeling and separation reactions.

Important Features of Microscale Protein Labeling Kits:

Labeled proteins typically ready to use typically in 2 hours (∼30 minutes hands-on time)

Optimized for 20–100µg of protein with molecular weights between 12 and 150 kDa

Purified using convenient spin filters with yields between 60 and 90%

Stabilizing proteins must be removed from the sample before labeling

Stable Reaction Chemistry and Superior Alexa Fluor™ Dyes
In the Microscale Protein Labeling Kits, the reactive dye contains a succinimidyl (NHS) ester moiety that reacts with primary amines of proteins to form stable dye-protein conjugates. Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. And generally when using Alexa Fluor™ dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor™ Dyes Spanning the Visible and Infrared Spectrum—Section 1.3.

Learn More About Protein and Antibody Labeling
We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See Antibody Labeling from A to Z or use our Labeling Chemistry Selection Tool for other choices. To learn more about our various kits read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in the Molecular Probes™ Handbook.

We’ll Make a Custom Antibody Conjugate for You
If you can’t find what you’re looking for in our stocked list, we’ll prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Product Type

Labeling Kit

Quantity

1 kit

Content And Storage

Store in refrigerator 2°C to 8°C and protect from light.

Detection Method

Fluorescence

Excitation/Emission

590/617 nm

Labeling Method

Conjugation-based

Labeling Scale

20–100 μg

Shipping Condition

Room Temperature

Product Line

Alexa Fluor™

Reactive Moiety

Succinimidyl (NHS) Ester

Label or Dye

Alexa Fluor 594

Color

Red

Labeling Target

Proteins

Label Type

Alexa Fluor

For Research Use Only. Not for use in diagnostic procedures.