Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit

Makes it possible to perform expression analysis directly from cultured cells without RNA purification

Supplier:  Invitrogen™ A25599

Catalog No. A25599

Description

The one-step format of RT-PCR step streamlines the Cells-to- CT “sample to answer” workflow more than ever without compromising sensitivity and specificity and at extraordinary value.

• Complete: optimized workflow includes cell lysis reagents with gDNA removal and Power SYBR Green 1-Step RT-PCR Master Mix
  Faster: 7-minute sample prep, including DNase treatment, versus 30-60 minutes for traditional RNA purification
• Simple: 12 steps total (sample prep and real-time RT-PCR combined)
• Robust: Perform gene expression analysis on 10 - 100,000 cells per sample; results equivalent to those from purified RNA
• Study multiple targets per sample at an affordable price

Accessory Product: The SYBR Green Cells-to-CT Control Kit was designed for use with SYBR Green Cells-to-CT products. This kit contains an endogenous control primer set (ACTB) to normalize for sample loading variability and an artifical XenoRNA control and corresponding primer set to monitor the effects of PCR inhibition.

Gene Expression Analysis & Genotyping, Real Time PCR-Based Gene Expression Profiling

Specifications

• Content And Storage: 22 mL Lysis Solution, store at 2°C to 8°C, 220 μL Dnase I, store at -5° to -30°C, 2 x 1 mL Stop Solution, store at -5° to -30°C, 20 mL Power SYBR™ Green 1-step qPCR Mix, store at -5° to -30°C, 320 μL Power SYBR™ Green 1-step RT Mix, store at -5° to -30°C
• Format: Tube
• PCR Method: 1-step RT-qPCR
• For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
• Product Line: Cells-to-CT™, Power SYBR™ Green
• Quantity: 400 Preps
• Reagent Type: Cell Lysis
• Starting Material: 10-100,000 Cells

• Detection Method: SYBR
• GC-Rich PCR Performance: High
• Reaction Speed: 7 min.
• Green Features: Fewer resources used and less waste, less hazardous
• Product Type: 1-Step qPCR kit
• For Use With (Application): Gene Expression
• Sample Type: Mammalian Cells
• Volume: 22 mL

For Research Use Only. Not for use in diagnostic procedures.