Invitrogen™ LeukoLOCK™ Fractionation & Stabilization Kit with Manual

Includes

RNAlater Solution, Rubber Septum Caps, White Slip Connectors, Transfer Spikes, LeukoLOCK Filters, and Elution Solution, and 1X PBS

The Ambion™ LeukoLOCK™ Fractionation & Stabilization Kit is one of two kits that comprise the Total RNA Isolation System (patent pending), an innovative method for cellular fractionation of whole blood and total RNA stabilization and extraction from the leukocyte population.

The Ambion™ LeukoLOCK™ Fractionation & Stabilization Kit is one of two kits that comprise the Total RNA Isolation System (patent pending), an innovative method for cellular fractionation of whole blood and total RNA stabilization and extraction from the leukocyte population. The kit includes sufficient reagents for 20 reactions. The LeukoLOCK™ System (Cat. No. AM1923) is optimized for use with human blood. Blood is a storehouse of cellular information; however, the presence of globin mRNA in RNA prepared from whole blood can interfere with downstream expression profiling applications. The LeukoLOCK™ System employs filter-based leukocyte-depletion technology to isolate leukocytes from whole blood and Ambion's RNAlater™ Solution to stabilize the cells on the filter. By excluding red blood cells, the RNA that is purified from captured leukocytes is inherently depleted of globin mRNA, which improves sample utility for expression profiling and other applications (see Figure).

LeukoLOCK™ Fractionation & Stabilization Kit
A 9–10 mL sample of anticoagulated blood is passed through a LeukoLOCK™ filter, which captures the total leukocyte population while eliminating red blood cells (including reticulocytes), platelets, and plasma. After rinsing with phosphate-buffered saline, the filter is flushed with RNAlater™ Solutionto stabilize the RNA in the captured leukocytes (see protocol schematic). The RNA can be isolated immediately, or stabilized cells can be maintained for several days at room temperature or for longer periods at –20°C or –80°C (see Figure).

Accessory Products:
LeukoLOCK™ Fractionation & Stabilization Kit is designed for use with the LeukoLOCK™ Total RNA Isolation Kit (Cat. No. AM1934). These kits are offered separately to accommodate blood sample collection at one location and sample processing at a different location, but may be purchased together as the LeukoLOCK™ Total RNA Isolation System (Cat. No. AM1923).

Order Info

Shipping Condition: Room temperature

Concentration

1X

Content And Storage

The RNAlater™ Solution, Rubber Septum Caps, White Slip Connectors, Transfer Spikes, LeukoLOCK™ Filters, and Elution Solution, should be stored at room temperature. 1X PBS may be stored at any temperature.

Isolation Technology

Vacuum Filter Column, Magnetic Bead

Sample Type

Blood

Final Product Type

Cells (Leukocytes)

High-throughput Compatibility

Not High-throughput Compatible (Manual)

For Use With (Application)

Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting

Includes

RNAlater Solution, Rubber Septum Caps, White Slip Connectors, Transfer Spikes, LeukoLOCK Filters, and Elution Solution, and 1X PBS

Prep Scale

> 1 mL Blood Samples (Large-scale)

Product Line

Ambion™, LeukoLOCK™

Product Type

Kit

Shipping Condition

Room Temperature

Starting Material Amount

Up to 10 ml whole blood

Target

Total RNA

Yield

20 μg

For Research Use Only. Not for use in diagnostic procedures.