Invitrogen™ MEGAshortscript™ T7 Transcription Kit

Maximize the yield of short RNA transcripts

The MEGAshortscript T7 Kit is designed for high yields of in vitro-transcribed RNA products, in the 20 to 500 nucleotide range. Using conventional methods, transcription of small templates often results in low mass yields of RNA. Short transcripts require many more transcription initiation events to synthesize a given mass of product. Since the initiation step is the rate-limiting step in transcription reactions, the yield of product from short templates is often low. The MEGAshortscript T7 Kit has been designed to overcome the drawbacks involved in conventional in vitro transcription to maximize the yield of short RNA transcripts.

• Efficiently transcribes small templates (<300 bases) with consistently high yields
• Designed for use with plasmid, synthetic oligonucleotide, or PCR product templates

Content And Storage

T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-RNA 18S, TURBO DNase, Ammonium Acetate Stop Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.

Final Product Type

Synthetic mRNA, mRNA

Format

Kit

Sample Type

mRNA, Synthetic mRNA

For Use With (Application)

In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products

No. of Reactions

25 Reactions

Product Line

Ambion™, MEGAshortscript™

Product Type

T7 In Vitro Transcription kit

Quantity

25 Reactions

Promoter

T7

Shipping Condition

Dry Ice

Starting Material

PCR Products, Linearized DNA plasmid

For Research Use Only. Not for use in diagnostic procedures.