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Sufficient markers are provided for 25 northern gel lanes. They are a mixture of 10 discrete RNA transcripts with easy-to-remember sizes: 0.5, 1, 1.5, 2, 2.5, 3, 4, 5, 6, and 9 kilobases. The markers may be stained with ethidium bromide either during or after electrophoresis. Because of their quantity and even spacing, these markers are ideal for constructing very accurate standard curves for mRNA size determination. They also provide a reference scale for easy verification and comparison of known bands. 1–2μg (1–2μg) of RNA Millennium™ Markers will generate 10 distinct bands on a 1% denaturing agarose gel with ethidium bromide staining. All marker lots undergo stringent nuclease testing and are shown to be stable overnight at 37°C. Compatible with denaturing agarose and formaldehyde-containing gels.
Agarose Gel Electrophoresis, DNA and RNA Purification and Analysis, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting
Order Info
Shipping Condition: Dry Ice
Specifications
Content And Storage
Store at below –70°C.
Concentration
1 μg/μl
Product Type
RNA Marker
Ready to Load
No
Size Range
0.5 to 9 kb
Gel Compatibility
Formaldehyde-Containing Gels, Denaturing Agarose Gels
Product Line
Ambion™, Millennium Markers™
Quantity
50 μL
Shipping Condition
Dry Ice
For Research Use Only. Not for use in diagnostic procedures.
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