Invitrogen™ mMESSAGE mMACHINE™ SP6 Transcription Kit

in vitro synthesis of large amounts of capped RNA

mMESSAGE mMACHINE™ kits are designed for in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5′ end.

• RNA Yield: 5-25μg
• Include cap analog [m7G(5′)ppp(5′)G] in an ultra high-yield transcription reaction
• Cap analog is incorporated only as first or 5′ terminal G of transcript because its structure precludes its incorporation at other position in RNA molecule
• Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
• Ideal for routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications

Content And Storage

SP6 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.

Final Product Type

Synthetic mRNA, mRNA

Format

Kit

Sample Type

mRNA, Synthetic mRNA

For Use With (Application)

In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products

No. of Reactions

25 Reactions

Product Line

Ambion™, mMESSAGE mMACHINE™

Product Type

SP6 In Vitro Transcription kit

Promoter

SP6

Shipping Condition

Dry Ice

Yield

15-25 μg

Starting Material

PCR Products, Linearized DNA plasmid

For Research Use Only. Not for use in diagnostic procedures.