Invitrogen™ mMESSAGE mMACHINE™ T7 Transcription Kit

in vitro synthesis of large amounts of capped RNA

mMESSAGE mMACHINE™ kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5′ end.

• RNA Yield: 20-30μg
• Include cap analog [m⁷G(5′)ppp(5′)G] in ultra high-yield transcription reaction
• Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
• Cap analog: GTP ratio of solution is 4:1, which is optimal for maximizing both RNA yield and the proportion of capped transcripts
• For routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications
• High yields are achieved by optimizing reaction conditions for RNA synthesis in presence of high nucleotide concentrations
• RNA synthesized is protected from degradation by contaminating ribonucleases that present with RNase inhibitor - component of the Enzyme Mix

Content And Storage

T7 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.

Final Product Type

Synthetic mRNA, mRNA

Format

Kit

Sample Type

mRNA, Synthetic mRNA

For Use With (Application)

In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products

No. of Reactions

25 Reactions

Product Line

Ambion™, mMESSAGE mMACHINE™

Product Type

T7 In Vitro Transcription kit

Quantity

25 Reactions

Promoter

T7

Shipping Condition

Dry Ice

Yield

20-30 μg

Starting Material

PCR Products, Linearized DNA plasmid

For Research Use Only. Not for use in diagnostic procedures.