Invitrogen™ Poly(A)Purist™ MAG Kit

Includes

Store Nuclease–free Water, Microfuge Tubes and Microfuge tubes with Spin Columns at room temperature. Store 2X Binding Solution, Wash Solution 1, Wash Solution 2 and Oligo(dT) Cellulose at 4°C. Store RNA Storage Solution, 5M Ammonium Acetate and Glycogen(5mg/mL) at -20°C.

Requires

Magnetic stand

Ambion™ Poly(A)Purist™ MAG Kit (patent pending) uses magnetic technology for the selection of mRNA that typically requires only a single round of selection yet yields RNA that is more highly enriched and devoid of rRNA than other procedures.

Ambion™ Poly(A)Purist™ MAG Kit (patent pending) uses magnetic technology for the selection of mRNA that typically requires only a single round of selection yet yields RNA that is more highly enriched and devoid of rRNA than other procedures. Each kit contains sufficient reagents for the isolation of mRNA from up to 8 mg of total RNA.

• Highest recovery of mRNA of any available kit
• High stringency for lowering residual rRNA content
• Single round of selection sufficient for most applications

The superior performance of Poly(A)Purist™ MAG Kit is clearly demonstrated by a comparison of the mRNA recovered using 4 other kits (see Figure). The kit uses batch binding of RNA to premeasured aliquots of oligo(dT) cellulose to circumvent the problems of slow flow rates and clogged columns often experienced during conventional, gravity-driven chromatography. The magnetic bead–based selection employed in the delivers increased processing speed, ease of handling, and scalability. The entire selection procedure can be completed in as little as 45 minutes (see Figure).

Accessory Products:
A magnetic stand is required for use this kit. They are available in several formats, including the Single Place Magnetic Stand (Cat. No. AM10026) and the 6 Tube Magnetic Stand (Cat. No. AM10055).

User Guide Alert (July 2023): Step 3 of the Preparation of Oligo (dT) MagBeads section (page 7) of the User Guide is misleading. Please follow this instruction for Step 3: add an equivalent mass of Oligo (dT) Magbeads into the selected empty tube and then continue with Oligo (dT) Magbeads preparation according to steps 4-9.

Order Info

Shipping Condition: Room Temperature

Content And Storage

Store Nuclease-free Water, Microfuge Tubes and Microfuge tubes with Spin Columns at room temperature. Store 2X Binding Solution, Wash Solution 1, Wash Solution 2 and Oligo(dT) Cellulose at 4°C. Store RNA Storage Solution, 5M Ammonium Acetate and Glycogen(5 mg/mL) at -20°C.

Format

Kit

Isolation Technology

Magnetic Bead, Oligo dT Affinity

Sample Type

Total RNA

Final Product Type

mRNA

High-throughput Compatibility

Not High-throughput Compatible (Manual)

For Use With (Application)

Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), Microinjection, Nucleic Acid Labeling, In Vitro Translation, cDNA Library Construction, Northern Blotting, Nuclease Protection Assays

Product Line

Ambion™, Poly(A)Purist™

Product Type

MAG Kit

Shipping Condition

Room Temperature

Target

mRNA

Test Time

45 min.

For Research Use Only. Not for use in diagnostic procedures.