Invitrogen™ PureLink™ miRNA Isolation Kit

The Invitrogen PureLink miRNA Isolation Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total microRNA (miRNA) from a wide variety of sources without the need for hazardous reagents such as phenol.

The Invitrogen PureLink miRNA Isolation Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total microRNA (miRNA) from a wide variety of sources without the need for hazardous reagents such as phenol. The kit provides a rapid and convenient method for purifying small RNA molecules from animal and plant cells, as well as from bacteria and yeast samples.

• Purify small RNA molecules with a novel enrich/bind/elute procedure
• Prepare enriched microRNA samples typically in less than 15 minutes
• Minimize contaminating large RNA molecules and genomic DNA

Rapid and Convenient Column Purification Procedure for small RNAs
The Invitrogen PureLink miRNA Isolation Kit is specifically designed to purify small RNA molecules, including miRNAs and siRNAs, from a variety of samples typically in less than 15 minutes. The kit utilizes a silica-based two-column system to enrich exclusively for small RNAs. RNAs greater than approximately 200 nucleotides are bound to the first column. Ethanol is then added to the flow-through in order to increase the binding affinity of small RNAs to the glass fiber membrane in the second column. Once bound to the second column, small RNA molecules are then eluted, ready-for-use, and free of large RNA molecules such as 28S rRNA, 18S rRNA, and mRNA, allowing for focused expression analysis studies of small RNA molecules

Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
The miRNA purified using the Invitrogen PureLink miRNA Isolation Kit is suitable for downstream applications including RNA sequencing, microarray analysis, real-time PCR (qPCR), reverse transcription real-time PCR (RT-qPCR), and northern blotting

Not high-throughput compatible (Manual)

Applications
DNA and RNA Purification and Analysis, RNA Extraction, RNAi, RNAi, Epigenetics and Non-Coding RNA Research, miRNA and Non-Coding RNA Analysis, miRNA Isolation, microRNA Isolation, siRNA

Order Info

Shipping Condition: Room temperature

Content And Storage

• 45 mL Binding Buffer L3; store at room temperature
• 10 mL Wsh Buffer W5; room temperature
• 3 mL Buffer W4; room temperature
• 5 mL RNase-free Water; room temperature
• 50 Spin Cartridges with Collection Tubes; room temperature
• 50 Wash Tubes (2 mL); room temperature
• 50 Recovery Tubes (1.7 mL); room temperature

Isolation Technology

Silica Spin Column

Purification Time

20 min. (After sample homogenization)

Elution Volume

50–100 μL

Sample Type

Bacteria, Cells, Plant, Tissue, Yeast, Dicing Reaction

Final Product Type

Small RNA

High-throughput Compatibility

Not High-throughput Compatible (Manual)

For Use With (Application)

qRT-PCR, Microarray Analysis, Northern Blotting

Quantity

25 preps

Scale

Mini

Shipping Condition

Room Temperature

Starting Material Amount

Bacteria: ≤10⁶ cells
Cells: 10⁶
Plant: varies
Tissue: ≤100 mg
Yeast: 1 mL
Dicing Reaction: 300 μL

Yield

Up to 6 μg

For Research Use Only. Not for use in diagnostic procedures.