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The Human Apolipoprotein E (Hu ApoE) ELISA quantitates Hu Apoe in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ApoE. Principle of the method The Human ApoE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Specifications
Accession Number
P02649
Assay Range
1.64-400 ng/mL
Assay Sensitivity
1.5 ng/mL
Calibration Range
1.5ng/mL to 400ng/mL
Description
Apolipoprotein E (AD2) ELISA Kit, 96 Assays, 1 Kit, Human Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Apolipoprotein E (AD2) in Samples Using a Microplate Reader
Detectable Analytes
Apolipoprotein E (AD2), human ApoE (isoforms ApoE2, ApoE3 and ApoE4)
Assay
Solid-phase sandwich ELISA
Conjugate
Biotin
Detection Method
Colorimetric
Product Type
ELISA Kit
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