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Invitrogen™ Apolipoprotein E Human ELISA Kit
SDP

Catalog No. p-7231027
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Sandwich ELISA Kit

The Human Apolipoprotein E (Hu ApoE) ELISA quantitates Hu Apoe in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ApoE. Principle of the method The Human ApoE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Apo E (Apolipoprotein E) plays an important role in the metabolism of lipids in the plasma, and is also is a constituent of various plasma lipoprotein-lipid particles. The protein is synthesized in liver, brain, spleen, and the kidney, and concentrations are high in interstitial fluids, where it plays a role in distributing cholesterol from cells where cholesterol is in excess to cells which require cholesterol. Apo E also plays an important role in the formation of very low density lipoprotein and chylomicrons. The Apo E gene is located on chromosome 19 at position 19q13.2.

Specifications

Accession Number

P02649

Assay Range

1.64-400 ng/mL

Assay Sensitivity

1.5 ng/mL

Calibration Range

1.5ng/mL to 400ng/mL

Description

Apolipoprotein E (AD2) ELISA Kit, 96 Assays, 1 Kit, Human Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Apolipoprotein E (AD2) in Samples Using a Microplate Reader

Detectable Analytes

Apolipoprotein E (AD2), human ApoE (isoforms ApoE2, ApoE3 and ApoE4)

Assay

Solid-phase sandwich ELISA

Conjugate

Biotin

Detection Method

Colorimetric

Product Type

ELISA Kit

Protein Family

GPCR (G-Protein Coupled Receptors)

Sample Type

Plasma, Serum, Supernatant

Specificity

This ELISA pair antibody detects human ApoE (isoforms ApoE2, ApoE3 and ApoE4).

Sufficient For

96 Assays

Target Species

Human

For Use With (Application)

ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis

For Use With (Equipment)

Colorimetric Microplate Reader

Gene ID (Entrez)

348

Gene Symbol

AD2,APO-E,ApoE4,LDLCQ5,LPG

Host Species

Human

Interassay CV

<12%

Intraassay CV

<10%

Kit Contents

Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-human Apo E precoated 96-well strip plate, lyophilized recombinant human Apo E standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent (5X concentrat

Label or Dye

HRP

Quantity

96 Tests

Regulatory Status

RUO

Research Discipline

Cardiovascular Research

Sample Volume

Plasma, 1 μL; Serum, 1 μL; Supernatant, 100 μL

Storage Requirements

2°C to 8°C

Synonym

AD2, APO-E, LDLCQ5, LPG

Target

Human

Test Time

1 hr. 20 min.

Total Assay Time

4 hr. 45 min.

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