Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase, LD (Low DNA) with Gold Buffer and MgCl₂

Applied Biosystems AmpliTaq Gold DNA Polymerase, LD (Low DNA) is a highly purified version of AmpliTaq Gold DNA Polymerase that significantly reduces contaminating bacterial DNA sequences commonly present in recombinant protein preparations.

Supplier:  Applied Biosystems™ 4338857

Catalog No. 43-388-57

Includes: Contains AmpliTaq Gold DNA Polymerase, LD (250 units), GeneAmp 10X PCR Gold Buffer, and MgCl₂ Solution.

Description

Applied Biosystems AmpliTaq Gold DNA Polymerase, LD (Low DNA), is a highly purified version of AmpliTaq Gold DNA Polymerase that significantly reduces contaminating bacterial DNA sequences commonly present in recombinant protein preparations.

This highly purified enzyme preparation gives you more confidence in your results and makes advanced PCR techniques quick and easy. When combined with the supplied GeneAmp 10X PCR Gold Buffer, AmpliTaq Gold DNA Polymerase, LD, provides a flexible hot-start system that is suitable for all PCR applications that require the elimination of bacterial DNA contamination.

Features

• Highly purified enzyme with lower levels of bacterial DNA
• Minimized non-target, false-positive PCR products when amplifying bacterial sequences
• Chemically modified hot-start technique eliminates biological contamination
• 10X PCR Gold Buffer promotes fast enzyme activation and robust PCR amplification
• Enhanced specificity with time-release PCR for low-copy target amplification
• Improved yield and success rate for target amplification

Notes

• AmpliTaq Gold DNA Polymerase, LD, is recommended for PCR applications that require low background levels of bacterial DNA.
• See user's manual or package insert for limited label license and trademark information.

Specifications

• Content And Storage: • AmpliTaq Gold DNA Polymerase, LD (5 U/μL), 1 x 200 μL
  • 10X PCR Gold Buffer, 4 x 1.5 mL
  • 25 mM MgCl₂, 4 x 1.5 mL
  
  Store at -15°C to -30°C.
• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Product Type: DNA Polymerase
• Quantity: 1000 Units
• For Use With (Application): Hot-start PCR
• Fidelity (vs. Taq): 1X
• No. of Reactions: 800 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

• Format: Tube
• Polymerase: AmpliTaq Gold DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Purity or Quality Grade: LD (Low DNA)
• Shipping Condition: Dry Ice
• Concentration: 5 U/μL
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

For Research Use Only. Not for use in diagnostic procedures.