Arsenazo III, Thermo Scientific Chemicals

Arsenazo III (3,6-bis((E)-(2-Arsonophenyl)diazenyl)-4,5-dihydroxynaphthalene-2,7-disulfonic acid, CAS # 1668-00-4) is a metallochrome indicator that is used for detecting calcium levels in plasma and serum.

Suitable for determination of micromolar amounts of calcium

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• Arsenazo III is highly specific for calcium and produces absorbance peaks in the 600-660 nm range
• It reacts in slightly acidic conditions, and is soluble in alcohol and semi-soluble in water

Application

• Arsenazo III can detect calcium in permeabilized cells; it is also capable of detecting uranium and thorium
• Arsenazo III has been used in vitro to measure intracellular Ca2+ levels in live neurons alongside patch clamp methods

Chemical Identifiers

CAS

1668-00-4

Molecular Formula

C22H18As2N4O14S2

Molecular Weight (g/mol)

776.363

MDL Number

MFCD00036695

InChI Key

TVMZRHVOFZTNET-RIRMOVKSSA-N

Synonym

arsenazo iii, arsenazo iii sodium salt, 2,2'-1, 8-dihydroxy-3,6-disulfo-2, 7-naphthalene-bis azo dibenzenearsonic acid, 2,7-bis 2-arsonophenylazo-1, 8-dihydroxy-3, 6-naphthalene disulfonic acid, 2,7-bis 2-arsonophenylazo-1,8-dihydroxynaphthalene-3,6-disulfonic acid, 2,7-bis 2-arsonophenylazo chromotropic acid, 2,2'-1,8-dihydroxy-3,6-disulfonaphthylene-2,7-bisazo bisbenzenearsonic acid, 3,6-bis 2-2-arsonophenyl diazen-1-yl-4,5-dihydroxynaphthalene-2,7-disulfonic acid, 3,6-bis 2-2-arsonophenyl diazenyl-4,5-dihydroxy-2,7-naphthalenedisulfonic acid, 3,6-bis 2-2-arsonophenyl hydrazin-1-ylidene-4,5-dioxonaphthalene-2,7-disulfonic acid

PubChem CID

9810878

IUPAC Name

(3Z,6E)-3,6-bis[(2-arsonophenyl)hydrazinylidene]-4,5-dioxonaphthalene-2,7-disulfonic acid

SMILES

C1=CC=C(C(=C1)NN=C2C(=CC3=C(C2=O)C(=O)C(=NNC4=CC=CC=C4[As](=O)(O)O)C(=C3)S(=O)(=O)O)S(=O)(=O)O)[As](=O)(O)O

Color

Brown to Purple

Quantity

25 g

UN Number

UN3465

Beilstein

5717957

Formula Weight

776.37

Physical Form

Powder

Chemical Name or Material

Arsenazo III

Hazard Category

H301+H331

Hazard Statement

GHS H Statement
H301-H331
Toxic if swallowed.
Toxic if inhaled.

Precautionary Statement

P261-P264b-P270-P271-P301+P310-P304+P340-P311-P330-P501c

DOTInformation

Transport Hazard Class: 6.1; Packing Group: III; Proper Shipping Name: ORGANOARSENIC COMPOUND, SOLID, N.O.S.

EINECSNumber

216-788-6

TSCA

No

Recommended Storage

Ambient temperatures

SDS

Hazard Category

H301+H331

Hazard Statement

GHS H Statement
H301-H331
Toxic if swallowed.
Toxic if inhaled.

Precautionary Statement

P261-P264b-P270-P271-P301+P310-P304+P340-P311-P330-P501c

DOTInformation

Transport Hazard Class: 6.1; Packing Group: III; Proper Shipping Name: ORGANOARSENIC COMPOUND, SOLID, N.O.S.

EINECSNumber

216-788-6

TSCA

No

Recommended Storage

Ambient temperatures

SDS

RUO – Research Use Only