MilliporeSigma™ Supelco™ Ascentis™ Express F5 UHPLC Column, 2 μm Particle Size

Description

Ascentis™ Express 2.0 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Ascentis™ Express 2.0 μm F5 is a high-speed, UHPLC column based on a Fused-Core™ particle design. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4 μm thick porous shell and the small overall particle size of 2 μm. The bonded, endcapped, dimethylpentafluorophenylpropyl stationary phase of Ascentis™ Express 2.0 μm F5 provides a stable, reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Having polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes).

Specifications

• Diameter (Metric): 2.1 mm
• Length (Metric): 2 cm
• Particle Size: 2 μm
• pH: 2 to 9
• Pore Size: 90 Å
• Stationary Phase: PFP (Pentafluorophenyl)
• Product Line: Ascentis
• Matrix: PFP (Pentafluorophenyl) Phase/Fused-Core Particle Platform
• Endcapped: Yes
• Phase: Reversed Phase
• Type: LC-MS Column
• Technique: Fused-Core UHPLC

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC. Fused-Core is a registered trademark of Advanced Materials Technology, Inc.