BD Annexin V

For flow cytometric analysis of cells that are undergoing apoptosis

Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, condensation of cytoplasm and nucleus, and internucleosomal cleavage of DNA. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from inner to outer leaflet of plasma membrane, thereby exposing PS to external cellular environment

• 35-36 kDa Ca2+ dependent phospholipid-binding protein that has high affinity for PS
• Binds to cells with exposed PS
• May be conjugated to fluorochromes, such as fluorescein isothiocyanate (FITC), to serve as sensitive probe
• Typically used in conjunction with vital dye such as 7-Amino-Actinomycin
• Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide
• Format: Purified

Flow cytometry, Blocking

Concentration

0.5mg/mL

For Use With (Application)

Blocking Agent, Flow Cytometry

Formulation

Aqueous Buffered solution containing ≤0.09% Sodium Azide

Molecular Weight (g/mol)

35 to 36

Name

Annexin V

Quantity

0.1 mg

Storage Requirements

-80°C

Recombinant

Recombinant

Form

Solution

Research use only