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BD BrdU In-Situ Detection Kit
Determines proliferation rates and cell cycle kinetics in normal and cancerous tissues
Supplier: BD 550803
Alert:
Highly toxic, avoid contact with skin, eyes, and mucous membranes
Includes: Fixation Buffer, Diluent Buffer, BD™ Retrievagen A Antigen Retrieval Solution, biotinylated anti-BrdU antibody, ready-to-use Streptavidin-Horseradish peroxidase (SAv-HRP) Solution, DAB Buffer, DAB Chromogen, and Control Slides
Requires : BrdU for in vitro or in vivo labeling, H2O2 (30%) solution for blocking endogenous peroxidase, phosphate buffer saline (PBS)
Description
Cellular incorporation of BrdU during S phase can be readily detected by anti-BrdU-specific antibodies following membrane permeabilization. Subsequent quantitation of cells positive for BrdU is possible using routine methods for cell analysis
- Measures labeling indices of different cell populations
- Improved specific staining with minimal background
- Designed antigen retrieval solution unmask antigenic sites and preserve tissue morphology
- Enables study of proliferation state of phenotypically defined cells within the micro-environment of tissues
- Reaction: 50 slides
Flow Cytometry, Immunohistochemistry
Specifications
| 1 Kit |
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