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BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution

Catalog No. BDB554722 Shop All BD Biosciences Products
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1X solution can be used for the simultaneous fixation and permeabilization of cells prior to intracellular cytokine staining

Recommended Assay Procedure: For the stimulation of cells, various in vitro methods have been reported for stimulating cells to produce cytokines. Polyclonal activators have been particularly useful for inducing cytokine-producing cells. These activators include the following: concanavalin A, lipopolysaccharide, phorbol esters plus calcium ionophore or ionomycin, phytohaemaglutinin, staphylococcus enterotoxin B, and monoclonal antibodies directed against subunits of the TCR/CD3 complex (with or without antibodies directed against costimulatory receptors, such as CD28).

Specifications

For Use With (Application)

Can be used for the simultaneous fixation and permeabilization of cells prior to intracellular Cyto kine staining

pH Range

7.2

Content And Storage

Store undiluted at 4°C

Product Type

Fixation and permeabilization solution

Quantity

125mL

Format

Liquid

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