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BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit with BD GolgiStop™

Catalog No. BDB554715 Shop All BD Biosciences Products
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Includes

  • Fixation/Permeabilization solution (125mL)
  • BD Perm/Wash™ Buffer, 10x concentrate containing Fetal Bovine Serum (FBS) and saponin (100mL)
  • Protein transport inhibitor BD GolgiStop™ (containing Monensin)

Provides GolgiStop protein transport inhibitor containing monensin

The ex vivo addition of GolgiStop to in vitro- or in vivo -stimulated cells blocks their intracellular transport processes. This results in the accumulation of most cytokine proteins in the Golgi complex and thereby enhances cytokine staining signals.

  • GolgiStop reagent is provided for treating up to 1L of cell culture at a cell density of up to 2 × 106 cells/mL

Specifications

Product Type

Fixation/Permeabilization Reagent

Includes

125 mL fixation/permeabilization solution, perm/Wash™ buffer, fetal bovine serum (FBS) 10X and 100 mL saponin, protein transport inhibitor BD GolgiStop™

No. of Tests

250

Regulatory Status

RUO

Content And Storage

4°C

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