Perm Buffer IV (10x), BD

Description

For improved immunofluorescent staining and detection of certain intracellular phosphorylated proteins

BD Phosflow™ Perm Buffer IV (Perm Buffer IV) is intended for the permeabilization of paraformaldehyde-fixed single-cell suspensions prepared from lymphoid tissues, peripheral blood mononuclear cells (PBMC) and lysed whole blood cells.

• Treatment with buffer and subsequent washing with staining buffer enables access for fluorescent antibodies to stain intracellular molecules
• Buffer preserves light scattering properties of cells
• Providies capacity for intracellular immunofluorescent staining in preparation for Phosflow cytometric analysis

Flow Cytometry, Intracellular staining

Specifications

• Chemical Name or Material: Cell Permeabilization Buffer
• Concentration: 10X
• For Use With (Application): Permeabilization of paraformaldehyde-fixed single-cell suspensions prepared from lymphoid tissues, peripheral blood mononuclear cells and lysed whole blood cells, flow cytometry, intracellular staining
• Quantity: 50 mL

Research Use Only