Gibco™ BenchStable™ DMEM/F12

Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

BenchStable DMEM/F-12 has been engineered for storage at room temperature while supporting cellular proliferation and maximum cell densities comparable to conventional DMEM/F12 when supplemented with ≥10% fetal bovine serum (FBS).

This DMEM/F-12 is modified as follows:
• Optimized for room temperature stability
• With GlutaMAX, Phenol Red
• Without HEPEPS

Using BenchStable DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. DMEM/F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. BenchStable DMEM/F-12 contains no proteins, lipids, or growth factors and so requires supplementation with ≥10% fetal bovine serum (FBS).

Content And Storage

Store at room temperature. Protect from light.

Cell Line

MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts

Classification

Animal Origin-free

Concentration

1 X

Form

Liquid

Product Type

DMEM (Dulbecco's Modified Eagle Medium)/F-12

Serum Level

Standard Serum Supplementation

Sterility

Sterile-filtered

With Additives

High Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate

Without Additives

No HEPES

Green Features

Energy efficient, Fewer resources used, Sustainable packaging

Manufacturing Quality

cGMP-compliant under the ISO 9001: 2015 standard

Product Line

BenchStable™

Quantity

500 mL

Shipping Condition

Room Temperature

For Research Use Only. Not for use in diagnostic procedures.