Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit

Includes

This kit contains BigDye™ Direct PCR Master Mix, Sequencing Master Mix, M13 Fwd Primer, M13 Rev Primer, and Control DNA CEPH 1347-02, This kit contains sufficient reagents for 24 reactions

The BigDye™ Direct Cycle Sequencing Kit, part of the BigDye™ product family, is a complete kit for your PCR and Sequencing needs.

• A streamlined, simple procedure
• Performance of all sequencing steps in a single reaction tube
• Fast migration with excellent resolution starting at the 1st base after the gene-specific primer sequence
• Substantial time savings versus previous workflows

Order Info

1 Kit

Content And Storage

This kit contains BigDye™ Direct PCR Master Mix, Sequencing Master Mix, M13 Fwd Primer, M13 Rev Primer, and Control DNA CEPH 1347-02. Store all components at -15 to -25°C. PCR Master Mix should be stored at 4°C after first use. This kit contains sufficient reagents for 1000 reactions.

Description

BigDye™ Direct Cycle Sequencing Kit, 1000 rxns, 8-well Strips, 96-well Plate, Fluorescent Dye Terminator Sequencing Technique, 3, Use with 130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, GeneAmp™ 9700, Veriti™ Thermal Cycler

Format

96-well Plate, 8-well Strips

For Use With (Equipment)

3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, SeqStudio Genetic Analyzer, Veriti Thermal Cycler, 3130 Genetic Analyzer, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer

For Use With (Application)

Comparative Sequencing (Germline Mutations - 30:70 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Standard Sequencing, Gap Closure, Homopolymer A or T, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations -10:90 Heterozygotes)

No. of Reactions

1000

Product Line

BigDye™

Product Type

Direct Cycle Sequencing Kit

Shipping Condition

Dry Ice

Technique

Fluorescent Dye Terminator Sequencing

Template Compatibility

BAC DNA, Cosmids, Lambda DNA, PCR Amplicons (10:90 Heterozygotes), Plasmid DNA (≤15Kb), Genomic DNA (Bacterial), Fosmids, PCR Amplicons (50:50 Heterozygotes), PCR Amplicons (30:70 Heterozygotes)

For research use only