Thermo Scientific™ Imject™ EDC Blue Carrier™ Protein Spin Kit

Description

Easily prepare effective immunogens with the highly-soluble, mollusk-derived hemocyanin and complete hapten-carrier conjugation kit.

Thermo Scientific™ Blue Carrier Protein is a highly-soluble, mollusk-derived hemocyanin formulated in convenient package sizes and a complete kit for carbodiimide (EDC-mediated) hapten-carrier conjugation via amine and carboxyl groups.

Blue Carrier Protein is a purified preparation of Concholepas concholepas hemocyanin (CCH). The large protein exhibits most of the same immunogenic properties as the popular carrier protein, keyhole limpet hemocyanin (KLH). However, its signficantly better solubility provides greater flexibility in immunogen preparation protocols by allowing a broader range of buffer and pH conditions for coupling peptides, proteins and other haptens using classical crosslinkers such as glutaraldehyde, Sulfo-SMCC and EDC.

Highlights:

• Convenient packages – choose lyophilized Blue Carrier Protein or complete EDC conjugation kit

• High-yield conjugation – each molecule of CCH contains hundreds of primary amines available for coupling haptens via EDC or NHS ester crosslinkers

• Validated quality – the immunogenic carrier protein is specially purified to ensure uniform chemical structure and solubility for consistent performance

• Superior solubility – increased solubility compared to KLH allows Blue Carrier Protein to be used in a broader range of conjugation protocols to accommodate unusual or poorly soluble haptens

• Highly immunogenic – provides essentially the same level of immunogenicity and phylogenetic distance as keyhole limpet hemocyanin (KLH)

Properties of Blue Carrier Protein:

• Alternative names: Concholepas concholepas hemocyanin, CCH
• Molecular weight: Whole molecule: ∽8,000,000; Subunits: ∽400,000 and ∽350,000
• Absorption: maxima 280nm, 350nm and 558nm
• Extinction coefficient (Abs. 1mg/mL solution): 1.4 at 280nm; 0.35 at 346nm
• A280/A350 Ratio: ∽4.0
• Copper/protein ratio: ∽16 Cu²⁺/protein molecule
• Isoelectric point: 6.0
• Solubility: Soluble in aqueous buffers pH 7.0 to 8.0
• Stability: Stable in aqueous buffers (∽pH 7.2 without Ca²⁺ or Mg²⁺)

Includes:

Kit contains lyophilized Blue Carrier Protein (in MES buffer), EDC crosslinker, buffers and desalting columns to easily prepare ready-to-use immunogens for injection

Specifications

• Content And Storage: Sufficient For: 5 EDC conjugation reactions and complete immunogen preparations, each with up to 2 mg peptide
  • Imject Blue Carrier Protein (in MES buffer), 5 x 2 mg
  • Imject EDC Conjugation Buffer, 30 mL
  • EDC Crosslinker, 5 x 10 mg
  • Imject Purification Buffer Salts, 5 x 0.84 g
  • Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 5 columns
  
  Store at 4°C
• Description: Imject EDC Blue Carrier Protein Spin Kit
• Format: Kit
• Quantity: 5 x 2 mg Kit
• Product Line: Imject™

For Research Use Only. Not for use in diagnostic procedures.