Thermo Scientific™ BS3 (bis(sulfosuccinimidyl)suberate)

Crosslink amines to amines with BS3; composed of sulfo-NHS-ester groups separated by an 11.4A, water-soluble spacer arm that is useful for cell-surface protein crosslinking.

Thermo Scientific™ Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

Highlights:

• Crosslink cell-surface proteins before cell lysis and immunoprecipitation
• Identify receptor-ligand interactions by chemical crosslinking
• “Fix” protein interactions to allow identification of weak or transient protein interactions
• Identify near-neighbor protein interactions
• Protein crosslinking to create bioconjugates via single-step reactions
• Immobilize proteins onto amine-coated surfaces

Chemical Reactivity

Amine-Amine

Cleavable

No

Molecular Weight (g/mol)

572.43

PEGylated

No

Spacer Arm Length

11.4 Å

Content And Storage

Upon receipt store desiccated at 4°C.

Cell Permeability

No

Shipping Condition

Ambient

Product Line

Pierce™

Labeling Method

Chemical Labeling

Crosslinker Type

Homobifunctional

Reactive Moiety

Sulfo-NHS Ester

Spacer

Medium (10 to 30 Å)

Form

Powder

Quantity

50 mg

Solubility

Water

Format

Standard, Single-use, Premium-grade

Water Soluble

Yes

Product Type

Crosslinker

For Research Use Only. Not for use in diagnostic procedures.