MilliporeSigma™ Calbiochem™ TLR1/TLR2 Agonist II, CU-T12-9

Description

A diphenyl substituted imidazole based compound that directly and selectively targets TLR1/2 and induces their dimerization and activates TLR1 and 2 signaling leading to NF-κB and AP-1 activation ( K _{D = 182 and 478 nM, respectively; EC50}

A diphenyl substituted imidazole based compound that directly and selectively targets TLR1/2 and induces their dimerization and activates TLR1 and 2 signaling leading to NF-κB and AP-1 activation ( K _D = 182 and 478 nM, respectively; EC₅₀ = 52.9nM for TLR2 in HEK-Blue cells over-expressing hTLR2). Does not affect the dimerization of TLR2/TLR6 and exhibits poor affinity towards TLR3, TLR4, TLR5, TLR7 and TLR8. Shown to compete with Pam₃CSK₄ (Cat. No. 506350; K _i = 45.4nM) for binding to TLR1/2 interface and enhance heterodimerization. Up-regulates the expression of TLR1, TLR2, TNFα, IL-10, and iNOS in RAW 264.7 macrophages in a time-dependent manner. Shown to be non-toxic up to 100μM concentration.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Specifications

• Content And Storage: Protect from light
• Form: Powder
• Quantity: 10 mg
• Color: Yellow
• Description: 98% by HPLC
• Target Proteins: TLR1/TLR2
• Molecular Formula: C₁₇H₁₃F₃N₄O₂
• Solubility: DMSO