Invitrogen™ CBQCA Plus Protein Quantitation Kit

The CBQCA Plus Protein Quantitation Kit is a specialized tool designed for the accurate quantitation of proteins in solution.

The CBQCA Plus Protein Quantitation Kit is a specialized tool designed for the accurate quantitation of proteins in solution. This kit is a refined version of the CBQCA Protein Quantitation Kit (Cat. No. C6667), with a significant modification: potassium cyanide, a toxic compound, has been replaced with mandelonitrile, a less-toxic alternative. This change does not compromise the kit's performance, maintaining the same level of sensitivity and accuracy as its predecessor.

The CBQCA Plus Protein Quantitation Kit exhibits high sensitivity and is capable of detecting as little as 50 ng of bovine serum albumin (BSA) in an assay volume of 100–200 μL. This sensitivity allows for precise measurements even in low-protein samples. Moreover, the kit is compatible with detergents, a feature that enhances its versatility. This compatibility makes it suitable for accurate quantitation of proteins in the presence of lipids, membrane fractions, or detergents, as well as for lipoproteins and small peptides.

The kit includes all necessary components for performing 300–800 assays, depending on the concentration of substrate used. The resulting fluorescent product is maximally excited at 465 nm and has an emission maxima at 550 nm. It is designed for use with a fluorescence microplate reader, but can also be adapted for use with a standard fluorometer or minifluorometer after dilution of the reaction mixture.

Green Features

Less hazardous

Assay Range

50 ng to 150 μg

Content And Storage

Sufficient for: 300 to 800 assays

• Component A: ATTO-TAG CBQCA derivatization reagent, 5 mg
• Component B: Dimethylsulfoxide (DMSO), 1 mL
• Component C: Mandelonitrile, 20 mM in 4 mL DMSO
• Component D: Bovine serum albumin (BSA) standard, 4 mg
• BupH Borate Buffer Pack

Store in freezer (-5°C to -30°C) and protect from light.

Assay

Fluorescent Protein Assay

Detection Method

Fluorescence

Product Type

Protein Quantitation Assay

For Use With (Application)

Quantitating proteins in solution

For Use With (Equipment)

Microplate Reader, Fluorometer

Quantity

1 Kit

Shipping Condition

Wet Ice

Format

Kit

Excitation/Emission

465/550 nm

For Research Use Only. Not for use in diagnostic procedures.