Invitrogen™ CD163 Human ELISA Kit

Sandwich ELISA Kit

The Human CD163 ELISA quantitates Hu CD163 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD163. Principle of the method The Human CD163 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Further, CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

Accession Number

Q86VB7

Assay Range

32.77-8000 pg/mL

Assay Sensitivity

30 pg/mL

Calibration Range

30pg/mL to 8000pg/mL

Assay

Solid-phase sandwich ELISA

Conjugate

Biotin

Product Type

ELISA Kit

Protein Family

Other Proteins

Sample Type

Plasma, Serum, Supernatant

Specificity

In this sandwich immunoassay, no cross-reactivity with recombinant mouse CD163, rmCD6 or recombinant human CD6.

Sufficient For

96 assays

Target Species

Human

For Use With (Application)

ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis

For Use With (Equipment)

Colorimetric Microplate Reader

Gene ID (Entrez)

9332

Gene Symbol

M130,MM130,SCARI1

Interassay CV

<12%

Intraassay CV

<10%

Kit Contents

Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers

Label or Dye

HRP

Quantity

96 Tests

Regulatory Status

RUO

Research Discipline

Other Research

Sample Volume

Plasma, 2 μL; Serum, 2 μL; Supernatant, 100 μL

Storage Requirements

2°C to 8°C

Synonym

M130, MM130

Target

Human

Test Time

1 hr. 20 min.

Total Assay Time

4 hr. 45 min.