Invitrogen™ Chromatin Condensation & Membrane Permeability Dead Cell Apoptosis Kit with Hoechst 33342, YO-PRO™-1, and PI dyes, for flow cytometry

This product detects apoptotic cells with changes in nuclear chromatin condensation and plasma membrane permeability, using three nucleic acid stains: UV-excitable Hoechst 33342, green fluorescent YO-PRO™ dye, and propidium iodide.

This product detects apoptotic cells with changes in nuclear chromatin condensation and plasma membrane permeability, using three nucleic acid stains: UV-excitable Hoechst 33342, green fluorescent YO-PRO™ dye, and propidium iodide. The YO-PRO™ dye can enter apoptotic cells, whereas red-fluorescent propidium iodide (PI) cannot. Thus after staining with YO-PRO™-1 dye and PI, apoptotic cells show green fluorescence, and dead cells show primarily red fluorescence and some green fluorescence. Blue fluorescent Hoechst 33342 brightly stains the condensed chromatin of apoptotic cells and more dimly stains the normal chromatin of live cells. The staining pattern resulting from the simultaneous use of these three dyes makes it possible to distinguish normal, apoptotic and dead cell populations by flow cytometry or fluorescence microscopy.

Quantity

1 kit

Format

Kit

Product Type

Dead Cell Apoptosis Kit

Content And Storage

Contains 1 vial of Hoechst 33342, 1 vial of YO-PRO™-1, and propidium iodide.

Store in refrigerator (2–8°C) and protect from light.

Flow Cytometer Laser Lines

488 nm

Excitation/Emission

YO-PRO™-1: 491/509 cm, Hoechst 33342: 350/461 cm, PI: 535/617 cm

No. of Reactions

200 Reactions

Shipping Condition

Room Temperature

Product Line

Vybrant™, YO-PRO™

For Use With (Application)

Flow Cytometry

Conjugate

Hoechst 33342, Propidium Iodide, YO-PRO-1

For Use With (Equipment)

Flow Cytometer

Form

Solution

For Research Use Only. Not for use in diagnostic procedures.