Molecular Probes™ Click-iT™ Plus Alexa Fluor™ 647 Picolyl Azide Toolkit

Click-iT™ Plus Alexa Fluor™ 647 Picolyl Azide Toolkit

Click-iT™ Plus Alexa Fluor™ picolyl azide toolkits contain everything you need to perform copper-catalyzed click reactions with compounds that are sensitive to copper, while retaining all of the benefits of the standard azide/alkyne click reaction. Picolyl azides react efficiently with chelated copper, so free copper in the click reaction can be minimized, protecting against undesired copper side reactions with proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and even small molecules (e.g., phalloidin).

Flexible—optimize your own Click–iT™ reaction for the detection or labeling of any alkyne-containing biomolecule in vitro or in cells and tissue samples

Bright and sensitive—superior fluorescent Alexa Flour™ dyes combined with the efficiency of picolyl azide

Convenient—contains all the buffers and reagents needed to create the optimal click reaction

Each Click-iT™ Plus Picolyl Azide Toolkit includes 100μg of Alexa Fluor™ dye-picolyl azide and all of the reagents required to create a reaction cocktail with the optimal copper and chelate concentration for any click chemistry-based labeling or detection application. Guidelines for designing and optimizing the picolyl azide/alkyne click reaction of your choice are provided in the product manual. Additionally Click-iT™ Plus picolyl azides offer increased sensitivity and faster reaction times compared to standard azides using identical reaction conditions.

Description

Click-iT™ Plus Alexa Fluor™ 647 Picolyl Azide Toolkit, 5mg, Solid, 650nm Emission, 670nm Excitation, 1 Year Shelf Life, 2 to 8°C Storage Requirements, Less Hazardous Green Features, Used for Labeling Chemistry

For Use With (Application)

Labeling chemistry, cell analysis, click chemistries, glycoprotein analysis with click chemistry, metabolic labeling and nascent protein synthesis with click chemistry, cell proliferation with click chemistry, nascent RNA detection and capture with click

Kit Contents

1 x 100μg Alexa Fluor™ dye-picolyl azide, 1 x 4mL Click-iT™ reaction buffer, 1 x 1.5mL copper (II) sulfate, 1 x 500μL copper protectant, 1 Click-iT™ buffer additive

Product Type

Click-iT™ Plus Alexa Fluor™ 647 Picolyl Azide Toolkit

Quantity

1 Kit

Content And Storage

• 1 x 100 μg Alexa Fluor™ dye-picolyl azide
• 1 x 4 mL Click-iT™ reaction buffer
• 1 x 1.5 mL copper (II) sulfate
• 1 x 500 μL copper protectant
• 1 Click-iT™ buffer additive

Store at 2 to 8°C

Excitation/Emission

650/670

Product Line

Click-iT™

Label or Dye

Alexa Fluor 647

Format

Solid

For Research Use Only. Not for use in diagnostic procedures.