R&D Systems™ CometAssay Single Cell Gel Electrophoresis Assay-Silver

Includes

Lysis Solution, 2 x 500mL; Comet LMAgarose, 15mL; Trevigen CometSlide, 25/kit; pH 10 200mM EDTA, 12.5mL; Kit with Silver Stain (Mfr. No. 4251-050-K) also includes four staining reagents and a fixation additive.

Provides a simple and effective method for evaluating DNA damage in cells

The principle of the assay is based upon the ability of denatured, cleaved DNA fragments to migrate out of the nucleoid under the influence of an electric field, whereas undamaged DNA migrates slower and remains within the confines of the nucleoid when a

Choose the Neutral CometAssay to detect double-stranded breaks or the Alkaline CometAssay for a more sensitive protocol used to detect smaller amounts of damage including single and double-stranded breaks
Cells are immobilized in a bed of low melting poi

Quantity

50 Tests

Kit Contents

Lysis Solution, Comet LMAgarose, CometSlide, pH 10 200mM EDTA, four staining reagents and fixation additive, Silver Stain

Product Type

Single Cell Gel Electrophoresis Assay

Content And Storage

The product requires storage at room temperature, 2 - 8° C, and -20° C. Consult the product insert for specific storage temperatures. Do not use past expiration date.