BD CompBead Anti-Mouse Ig, κ/Negative Control Particles Set

Used to optimize fluorescence compensation settings for multicolor flow cytometric analyses. BD™ Anti-Mouse Ig, κ/Negative Control Particles Set includes two sets of polystyrene microparticles to distinctly stain positive and negative populations, especially when using tandem dye conjugates.

Supplier:  BD 552843

Catalog No. BDB552843

Description

The set provides two populations of microparticles:

• BD™ CompBeads Anti-Mouse Ig, κ—Binds any mouse κ light chain-bearing immunoglobulin
• BD™ CompBeads Negative Control—Has no binding capacity

When mixed together with a fluorochrome-conjugated mouse antibody, the CompBeads provide distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or using instrument set-up software.

Since the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies (without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra).

Use of the BD™ CompBeads is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy™7, APC-Cy™7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.

Specifications

• Antigen: Mouse Ig, κ
• Conjugate: Unconjugated
• Purification Method: Unpurified
• Primary or Secondary: Primary
• Form: Liquid

• Applications: Flow Cytometry
• Formulation: CompBead Anti-Mouse Ig, kappa Particles
  CompBead Negative Control (FBS) Compensation Particles
• Quantity: 6 mL Each
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.