Cytiva Con A Sepharose™ 4B Medium

An affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids

Highly suited for isolation of cell surface glycoproteins from detergent-solubilized membranes.

• Con A coupled to Sepharose, in a 4% agarose matrix via cyanogen bromide activation
• Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
• Group-specific adsorbent for molecules containing sugars
• Concentration of immobilized Con A, approx. 13mg/mL medium; binding capacity for porcine thyroglobulin, approx. 30mg/mL medium
• Stable in the pH range 4 to 9, and in the presence of all commonly used buffers
• Average particle size: 90μm
• Delivered in 0.1M acetate buffer, pH 6.0, containing 1M NaCl, 1mM CaCl₂, 1mM MgCl₂, 1mM MnCl₂, and 20% ethanol

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Binding Capacity

30mg/mL

Ligand Concentration

13mg Con A/mL Drained Medium

Matrix

4% Agarose

Particle Size

90 μm

pH

4 to 9

Quantity

5 mL

Content And Storage

2°C to 8°C

For Use With (Application)

For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes

Ligand

Concanavalin A