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HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2. Unlike the low pKa of bicarbonate, HEPES has a pKa of 7.66 making it a much stronger buffer in the pH 7.2-7.6 range than bicarbonate buffer. Potential disadvantages of HEPES include toxicity over 100 mM and lack of nutritive value to the cell. The recommended working concentration is 10-25 mM.
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Specifications
Content And Storage
15° to 30°C
Form
Liquid
Product Type
HEPES Buffer
Sterility
Sterile-filtered
pH
7.2 to 7.6
Quantity
6 x 100 mL
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