COS-7 (African green monkey kidney) Whole cell lysate, Non-denatured; Abnova

Description

• Tissue: Kidney; Sv40 transformed
• Host: African Green Monkey
• Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage buffer: In modified RIPA lysis buffer

Western Blotting, Immunoprecipitation

Specifications

• Tissue: Kidney, SV40-transformed
• For Use With (Application): Western Blot
• Description: Whole cell lysate (non-denatured)
• Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
• Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
• Quantity: 200 μg
• Storage Buffer: In modified RIPA Lysis Buffer.
• Host Species: African Green Monkey
• Concentration: 5 mg/mL
• Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.