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Invitrogen™ CSM Media for Mav203 Yeast Cells
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2L
Description
The CSM Media for Mav203 Yeast Cells is a specifically designed media for making agar plates that enable the growth of Saccharomyces cerevisae MaV203 cells. This product comes with enough reagents to prepare 2 liters of solid media with the following components per liter:
Compatible with All MaV203 Applications
The CSM Media is optimized for the GeneArt™ High-Order Genetic Assembly System, including the recovery for transformations of vectors once they have been adapted to yeast using the GeneArt™ High-Order Vector Conversion Cassette. The media can also be used to grow MaV203 competent yeast cells, both library and sub-cloning scale (cat# 11281-011 and 11445-012).
Plate Preparation Protocol
For each liter, use one pouch of CSM -Trp Agar Yeast Media minus Glucose and 1 bottle of 20% Glucose. Dissolve the contents of the pouch in 900mL of distilled water and autoclave for 20 minutes on liquid cycle. Allow the solution to cool to 55°C and then add one bottle of 20% Glucose (100mL). Mix well and pour plates.
For Research Use Only. Not for use in diagnostic procedures.
- CSM (without tryptophan): 0.74g
- Yeast Nitrogen Base: 6.66g
- Ammonium Sulfate: 5g
- Glucose: 20g
- Agar: 20g
Compatible with All MaV203 Applications
The CSM Media is optimized for the GeneArt™ High-Order Genetic Assembly System, including the recovery for transformations of vectors once they have been adapted to yeast using the GeneArt™ High-Order Vector Conversion Cassette. The media can also be used to grow MaV203 competent yeast cells, both library and sub-cloning scale (cat# 11281-011 and 11445-012).
Plate Preparation Protocol
For each liter, use one pouch of CSM -Trp Agar Yeast Media minus Glucose and 1 bottle of 20% Glucose. Dissolve the contents of the pouch in 900mL of distilled water and autoclave for 20 minutes on liquid cycle. Allow the solution to cool to 55°C and then add one bottle of 20% Glucose (100mL). Mix well and pour plates.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Specifications
| Content And Storage | CSM Media for MaV203 Yeast Cells has enough reagents to prepare 2 liters of agar media. The product contains two pouches of premixed powder and two 100 ml bottles of filter-sterilized, 20% glucose. Store all components at room temperature. All components are guaranteed stable for 6 months when properly stored. |
| Form | Liquid, Powder |
| Media Type | CSM Media |
| Preparation Method | Autoclave |
| Product Type | Microbiology Media |
| Sterility | Non-sterile |
| Target Organism Class | S. cerevisiae |
| Final Product Type | Agar Plates |
| For Use With (Application) | Optimized for use with the GENEART™ High-Order Genetic Assembly System, A13285 |
| Product Line | GeneArt™ |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
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