Edvotek™ Detecting COVID-19 Using Reverse-Transcription PCR (RT-PCR)

In this simulated RT-PCR experiment, students will explore the diagnostic test used worldwide to diagnose and monitor the spread of COVID-19.

• This experiment requires a PCR thermal cycler
• Please note, this experiment is a DNA simulation of reverse transcription PCR and is intended solely for educational purposes. There are no viral or human DNA products included in this experiment. The experiment will not perform viral RNA testing for SARS-CoV-2
• For 25 students working in 5 groups
• Time required: Set up - 30 minutes, PCR - 2 hours or overnight, Electrophoresis - 45 minutes
• Instructions, PCR EdvoBeads™, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, DNA Templates, TE Buffer, UltraSpec-Agarose™, TBE Electrophoresis Buffer powder, SYBR™ Safe Stain, FlashBlue™ DNA Stain, Microcentrifuge & PCR Tubes
• Some components require freezer storage upon receipt

Product Type

PCR Kit

Grade

9 to Undergraduate

Age

15 to 20

For Use With (Application)

Explore the diagnostic test used worldwide to diagnose and monitor the spread of COVID-19.

Includes

Instructions, PCR EdvoBeadsTM, LyphoPrimerTM Mix, EdvoQuickTM DNA Ladder, DNA Templates, TE Buffer, UltraSpec-AgaroseTM, TBE Electrophoresis Buffer powder, SYBR™ Safe Stain, FlashBlueTM DNA Stain, Microcentrifuge & PCR Tubes

Requires

DNA Electrophoresis, Thermal Cycler, Micropipettes: 5-50 μL, UV or Blue Light Transilluminator, White Light Box (optional - use if staining with FlashBlueTM), Microcentrifuge & Microwave or Hot plate.

Content And Storage

Some Components Require Freezer Storage Upon Receipt

Class Size

25 Students (5 Groups)

**Please note, this experiment is a DNA simulation of reverse transcription PCR and is intended solely for educational purposes. There are no viral or human DNA products included in this experiment. The experiment will not perform viral RNA testing for SARS-CoV-2.**